Product: OPRK1 Antibody
Catalog: DF5044
Description: Rabbit polyclonal antibody to OPRK1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse
Mol.Wt.: 48 KD; 43kD(Calculated).
Uniprot: P41145
RRID: AB_2837403

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%)
Clonality:
Polyclonal
Specificity:
OPRK1 Antibody detects endogenous levels of total OPRK1.
RRID:
AB_2837403
Cite Format: Affinity Biosciences Cat# DF5044, RRID:AB_2837403.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

K-OR-1; Kappa opioid receptor; Kappa type opioid receptor; Kappa-type opioid receptor; KOR 1; KOR; KOR-1; Opiate receptor kappa 1; Opiate receptor, kappa 1; Opioid receptor, kappa 1; OPRK 1; OPRK; OPRK_HUMAN; OPRK1; Oprk2; R21;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P41145 OPRK_HUMAN:

Detected in brain and placenta.

Sequence:
MDSPIQIFRGEPGPTCAPSACLPPNSSAWFPGWAEPDSNGSAGSEDAQLEPAHISPAIPVIITAVYSVVFVVGLVGNSLVMFVIIRYTKMKTATNIYIFNLALADALVTTTMPFQSTVYLMNSWPFGDVLCKIVISIDYYNMFTSIFTLTMMSVDRYIAVCHPVKALDFRTPLKAKIINICIWLLSSSVGISAIVLGGTKVREDVDVIECSLQFPDDDYSWWDLFMKICVFIFAFVIPVLIIIVCYTLMILRLKSVRLLSGSREKDRNLRRITRLVLVVVAVFVVCWTPIHIFILVEALGSTSHSTAALSSYYFCIALGYTNSSLNPILYAFLDENFKRCFRDFCFPLKMRMERQSTSRVRNTVQDPAYLRDIDGMNKPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41145 As Substrate

Site PTM Type Enzyme
N25 N-Glycosylation
N39 N-Glycosylation
S260 Phosphorylation
S262 Phosphorylation
K338 Ubiquitination
K349 Ubiquitination
S358 Phosphorylation Q15835 (GRK1)
Y369 Phosphorylation
K378 Ubiquitination

Research Backgrounds

Function:

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in brain and placenta.

Subunit Structure:

Interacts with SLC9A3R1. Interacts with GABARAPL1.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Co-Administration of nalbuphine to improve morphine tolerance in mice with bone cancer pain. Molecular Pain, 2023 (PubMed: 37226458) [IF=3.3]

Application: WB    Species: Mouse    Sample:

Figure 5. Changes of spinal MOR and KOR protein expression over time in tumor-implanted mice and sham-implanted mice. Western blot for β-actin, MOR, and KOR resulted in products of 42, 45, and 43 kDa, as expected (markers show predicted band sizes) (a and b). Densitometric quantification of β-actin, MOR, and KOR immunoreactivity on Western blots (c and d). Data were presented as fold change of control (Naive) mean ± SD.*p < 0.05 vs. sham-implanted mice. (n = 4 per group). (Interaction: F-statistics = 15.92/17.71; Row Factor: F-statistics = 43.83/25.90; Column Factor: F-statistics = 115.70/113.90, MOR protein/KOR protein respectively).

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