OPRK1 Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from COS-7 cells, using OPRK1 antibody.

Product:	OPRK1 Antibody
Catalog:	DF5044
Description:	Rabbit polyclonal antibody to OPRK1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse
Mol.Wt.:	48 KD; 43kD(Calculated).
Uniprot:	P41145
RRID:	AB_2837403

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%)

Clonality:

Polyclonal

Specificity:

OPRK1 Antibody detects endogenous levels of total OPRK1.

RRID:

AB_2837403
Cite Format: Affinity Biosciences Cat# DF5044, RRID:AB_2837403.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

K-OR-1; Kappa opioid receptor; Kappa type opioid receptor; Kappa-type opioid receptor; KOR 1; KOR; KOR-1; Opiate receptor kappa 1; Opiate receptor, kappa 1; Opioid receptor, kappa 1; OPRK 1; OPRK; OPRK_HUMAN; OPRK1; Oprk2; R21;

Immunogens

Immunogen:

Uniprot:

P41145(OPRK_HUMAN) >>Visit HPA database.

Gene(ID):

OPRK1(4986)

Expression:

P41145 OPRK_HUMAN:

Detected in brain and placenta.

Sequence:

P41145 OPRK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDSPIQIFRGEPGPTCAPSACLPPNSSAWFPGWAEPDSNGSAGSEDAQLEPAHISPAIPVIITAVYSVVFVVGLVGNSLVMFVIIRYTKMKTATNIYIFNLALADALVTTTMPFQSTVYLMNSWPFGDVLCKIVISIDYYNMFTSIFTLTMMSVDRYIAVCHPVKALDFRTPLKAKIINICIWLLSSSVGISAIVLGGTKVREDVDVIECSLQFPDDDYSWWDLFMKICVFIFAFVIPVLIIIVCYTLMILRLKSVRLLSGSREKDRNLRRITRLVLVVVAVFVVCWTPIHIFILVEALGSTSHSTAALSSYYFCIALGYTNSSLNPILYAFLDENFKRCFRDFCFPLKMRMERQSTSRVRNTVQDPAYLRDIDGMNKPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Pig

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41145 As Substrate

P41145

Site	PTM Type	Enzyme	Source
N25	N-Glycosylation		Uniprot
N39	N-Glycosylation		Uniprot
S260	Phosphorylation		Uniprot
S262	Phosphorylation		Uniprot
K338	Ubiquitination		Uniprot
K349	Ubiquitination		Uniprot
S358	Phosphorylation	Q15835 (GRK1)	Uniprot
Y369	Phosphorylation		Calculation
K378	Ubiquitination		Uniprot

Research Backgrounds

P41145_OPRK_HUMAN

Function:

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Detected in brain and placenta.

Subunit Structure:

Interacts with SLC9A3R1. Interacts with GABARAPL1.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Co-Administration of nalbuphine to improve morphine tolerance in mice with bone cancer pain. Molecular Pain, 2023 (PubMed: 37226458) [IF=3.3]

Application: WB Species: Mouse Sample:

Figure 5. Changes of spinal MOR and KOR protein expression over time in tumor-implanted mice and sham-implanted mice. Western blot for β-actin, MOR, and KOR resulted in products of 42, 45, and 43 kDa, as expected (markers show predicted band sizes) (a and b). Densitometric quantification of β-actin, MOR, and KOR immunoreactivity on Western blots (c and d). Data were presented as fold change of control (Naive) mean ± SD.*p < 0.05 vs. sham-implanted mice. (n = 4 per group). (Interaction: F-statistics = 15.92/17.71; Row Factor: F-statistics = 43.83/25.90; Column Factor: F-statistics = 115.70/113.90, MOR protein/KOR protein respectively).

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OPRK1 Antibody - #DF5044