Neurotensin Receptor 1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat cells, using NTR1 antibody.

Product:	Neurotensin Receptor 1 Antibody
Catalog:	DF5002
Description:	Rabbit polyclonal antibody to Neurotensin Receptor 1
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep
Mol.Wt.:	46 KD; 46kD(Calculated).
Uniprot:	P30989
RRID:	AB_2837361

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Bovine(88%), Horse(88%), Sheep(88%)

Clonality:

Polyclonal

Specificity:

Neurotensin Receptor 1 Antibody detects endogenous levels of total Neurotensin Receptor 1.

RRID:

AB_2837361
Cite Format: Affinity Biosciences Cat# DF5002, RRID:AB_2837361.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

High affinity levocabastine insensitive neurotensin receptor; Neurotensin receptor 1 (high affinity); Neurotensin receptor type 1; NT R 1; NTR; NTR1; NTR1_HUMAN; NTRH; NTRR; NTSR 1;

Immunogens

Immunogen:

Uniprot:

P30989(NTR1_HUMAN) >>Visit HPA database.

Gene(ID):

NTSR1(4923)

Expression:

P30989 NTR1_HUMAN:

Expressed in prostate (at protein level). Detected in colon and peripheral blood mononuclear cells. Detected at very low levels in brain.

Sequence:

P30989 NTR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRLNSSAPGTPGTPAADPFQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDIYSKVLVTAVYLALFVVGTVGNTVTAFTLARKKSLQSLQSTVHYHLGSLALSDLLTLLLAMPVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLAVPMLFTMGEQNRSADGQHAGGLVCTPTIHTATVKVVIQVNTFMSFIFPMVVISVLNTIIANKLTVMVRQAAEQGQVCTVGGEHSTFSMAIEPGRVQALRHGVRVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTPFLYDFYHYFYMVTNALFYVSSTINPILYNLVSANFRHIFLATLACLCPVWRRRRKRPAFSRKADSVSSNHTLSSNATRETLY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30989 As Substrate

P30989

Site	PTM Type	Source
K398	Ubiquitination	Uniprot
S401	Phosphorylation	Uniprot
S403	Phosphorylation	Uniprot
S404	Phosphorylation	Uniprot
T413	Phosphorylation	Uniprot
Y418	Phosphorylation	Uniprot

Research Backgrounds

P30989_NTR1_HUMAN

Function:

G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis.

PTMs:

N-glycosylated.

Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Membrane raft.
Note: Palmitoylation is required for localization at CAV1-enriched membrane rafts.

Tissue Specificity:

Expressed in prostate (at protein level). Detected in colon and peripheral blood mononuclear cells. Detected at very low levels in brain.

Subunit Structure:

Interacts (palmitoylated form) with GNA11.

Family&Domains:

The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembrane regions TM6 and TM7.

Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

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Neurotensin Receptor 1 Antibody - #DF5002