Product: NK1R Antibody
Catalog: DF4996
Description: Rabbit polyclonal antibody to NK1R
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50 KD; 46kD(Calculated).
Uniprot: P25103
RRID: AB_2837355

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NK1R Antibody detects endogenous levels of total NK1R.
RRID:
AB_2837355
Cite Format: Affinity Biosciences Cat# DF4996, RRID:AB_2837355.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Neurokinin 1 receptor; NK 1 receptor; NK 1R; NK-1 receptor; NK-1R; NK1 receptor; NK1R; NK1R_HUMAN; NKIR; SPR; Substance P receptor; Substance-P receptor; TAC 1R; TAC1R; Tachykinin 1 receptor (substance P receptor neurokinin 1 receptor); Tachykinin receptor 1 (substance P receptor neurokinin 1 receptor); Tachykinin receptor 1; TACR1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MDNVLPVDSDLSPNISTNTSEPNQFVQPAWQIVLWAAAYTVIVVTSVVGNVVVMWIILAHKRMRTVTNYFLVNLAFAEASMAAFNTVVNFTYAVHNEWYYGLFYCKFHNFFPIAAVFASIYSMTAVAFDRYMAIIHPLQPRLSATATKVVICVIWVLALLLAFPQGYYSTTETMPSRVVCMIEWPEHPNKIYEKVYHICVTVLIYFLPLLVIGYAYTVVGITLWASEIPGDSSDRYHEQVSAKRKVVKMMIVVVCTFAICWLPFHIFFLLPYINPDLYLKKFIQQVYLAIMWLAMSSTMYNPIIYCCLNDRFRLGFKHAFRCCPFISAGDYEGLEMKSTRYLQTQGSVYKVSRLETTISTVVGAHEEEPEDGPKATPSSLDLTSNCSSRSDSKTMTESFSFSSNVLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25103 As Substrate

Site PTM Type Enzyme
Y341 Phosphorylation
S347 Phosphorylation
S407 Phosphorylation

Research Backgrounds

Function:

This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ARRB1.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Viral > Measles.

References

1). Cinacalcet Targets the Neurokinin-1 Receptor and Inhibits PKCδ/ERK/P65 Signaling to Alleviate Dextran Sulfate Sodium-Induced Colitis. Frontiers in Pharmacology, 2021 (PubMed: 34880751) [IF=5.6]

Application: WB    Species: Mouse    Sample:

FIGURE 6 Cinacalcet suppresses the PKCδ/ERK/P65 signaling pathway. (A) Visualization of predicted the binding sites of cinacalcet with respect to NK1R. Structures on the yellow color and grey surface represent the NK1R structure, blue structures represent the amino acids of cinacalcet that bind to NK1R, and structures in pink represent structures of cinacalcet. (B) DARTs assay to test the binding of NK1R and cinacalcet. (C) Representative western blot results to show that cinacalcet suppresses the PKCδ/ERK/P65 signaling pathway. Bone marrow-derived macrophages (BMDMs) were starved with 2% FBS overnight. After treating the cells with cinacalcet (1 μM) for 2 h, TNFα (10 ng/ml) was added for different periods (0, 15, 30, 60 min). Proteins were extracted from the cells, and western blotting was performed. (D) Quantification of p-PKCδ expression. (E) Quantification of p-ERK expression. (F) Quantification of p-P65 expression. (G, H) qRT-PCR was performed to test the mRNA expression levels of IL-1β and IL-6 in BMDMs after stimulation with TNFα (10 ng/ml) in the presence or absence of cinacalcet for 24 h (I, J) ELISA was performed to detect IL-1β and IL-6 levels in BMDM supernatants after stimulation with TNFα (10 ng/ml) in the presence or absence of cinacalcet for 24 h. (K) Proposed model explaining the anti-TNF activity of cinacalcet through direct targeting of the NK1R pathway. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are shown as the mean with standard deviation. Three independent experiments were performed.

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