MTR1B Antibody - #DF4994

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Product: MTR1B Antibody
Catalog: DF4994
Description: Rabbit polyclonal antibody to MTR1B
Application: WB IF/ICC
Reactivity: Human, Rat
Mol.Wt.: 40 KD; 40kD(Calculated).
Uniprot: P49286
RRID: AB_2837353

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
MTR1B Antibody detects endogenous levels of total MTR1B.
RRID:
AB_2837353
Cite Format: Affinity Biosciences Cat# DF4994, RRID:AB_2837353.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FGQTL2; Mel 1b; Mel 1b melatonin receptor; Mel 1B R; MEL-1B-R; Mel1b; Mel1b melatonin receptor; Mel1b receptor; Mel1BR; melatonin receptor 1B; Melatonin receptor MEL 1B; Melatonin receptor MEL1B; Melatonin receptor type 1B; MT 2; MT 2 melatonin receptor; MT2; MT2 melatonin receptor; Mtnr 1b; Mtnr1b; MTR1B_HUMAN;

Immunogens

Immunogen:
Uniprot:

P49286(MTR1B_HUMAN) >>Visit HPA database.

Gene(ID):
MTNR1B(4544)
Expression:
P49286 MTR1B_HUMAN:

Expressed in retina and less in brain and hippocampus.

Sequence:
MSENGSFANCCEAGGWAVRPGWSGAGSARPSRTPRPPWVAPALSAVLIVTTAVDVVGNLLVILSVLRNRKLRNAGNLFLVSLALADLVVAFYPYPLILVAIFYDGWALGEEHCKASAFVMGLSVIGSVFNITAIAINRYCYICHSMAYHRIYRRWHTPLHICLIWLLTVVALLPNFFVGSLEYDPRIYSCTFIQTASTQYTAAVVVIHFLLPIAVVSFCYLRIWVLVLQARRKAKPESRLCLKPSDLRSFLTMFVVFVIFAICWAPLNCIGLAVAINPQEMAPQIPEGLFVTSYLLAYFNSCLNAIVYGLLNQNFRREYKRILLALWNPRHCIQDASKGSHAEGLQSPAPPIIGVQHQADAL

Research Backgrounds

Function:

High affinity receptor for melatonin. Likely to mediate the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in retina and less in brain and hippocampus.

Subunit Structure:

Interacts with GPR61, GPR62 and GPR135.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Environmental adaptation > Circadian entrainment.

References

1). Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function. JOURNAL OF LIPID RESEARCH, 2019 (PubMed: 30552289) [IF=6.5]

Application: WB    Species: pig    Sample: porcine intramuscular preadipocytes

Fig.?4.|Melatonin receptor (MT2) participates in lipolysis in precursor adipocytes. A: The mRNA levels of MT1 and MT2 mRNA were measured in melatonintreated mature adipocytes by RT-qPCR. B, C: Western blot analyses were performed to detect the changes of MT1 and MT2. D–F: RT-qPCR analysis of genes involved in lipolysis in mature adipocytes cultivated in GM, GM + melatonin, and GM + melatonin + 4-PPDOT medium for 48 h. Data are represented as the mean ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001; n = 3).

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