Product: MTR1B Antibody
Catalog: DF4994
Description: Rabbit polyclonal antibody to MTR1B
Application: WB IF/ICC
Reactivity: Human, Rat
Mol.Wt.: 40 KD; 40kD(Calculated).
Uniprot: P49286
RRID: AB_2837353

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
MTR1B Antibody detects endogenous levels of total MTR1B.
RRID:
AB_2837353
Cite Format: Affinity Biosciences Cat# DF4994, RRID:AB_2837353.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FGQTL2; Mel 1b; Mel 1b melatonin receptor; Mel 1B R; MEL-1B-R; Mel1b; Mel1b melatonin receptor; Mel1b receptor; Mel1BR; melatonin receptor 1B; Melatonin receptor MEL 1B; Melatonin receptor MEL1B; Melatonin receptor type 1B; MT 2; MT 2 melatonin receptor; MT2; MT2 melatonin receptor; Mtnr 1b; Mtnr1b; MTR1B_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P49286 MTR1B_HUMAN:

Expressed in retina and less in brain and hippocampus.

Sequence:
MSENGSFANCCEAGGWAVRPGWSGAGSARPSRTPRPPWVAPALSAVLIVTTAVDVVGNLLVILSVLRNRKLRNAGNLFLVSLALADLVVAFYPYPLILVAIFYDGWALGEEHCKASAFVMGLSVIGSVFNITAIAINRYCYICHSMAYHRIYRRWHTPLHICLIWLLTVVALLPNFFVGSLEYDPRIYSCTFIQTASTQYTAAVVVIHFLLPIAVVSFCYLRIWVLVLQARRKAKPESRLCLKPSDLRSFLTMFVVFVIFAICWAPLNCIGLAVAINPQEMAPQIPEGLFVTSYLLAYFNSCLNAIVYGLLNQNFRREYKRILLALWNPRHCIQDASKGSHAEGLQSPAPPIIGVQHQADAL

Research Backgrounds

Function:

High affinity receptor for melatonin. Likely to mediate the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in retina and less in brain and hippocampus.

Subunit Structure:

Interacts with GPR61, GPR62 and GPR135.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Environmental adaptation > Circadian entrainment.

References

1). Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function. JOURNAL OF LIPID RESEARCH, 2019 (PubMed: 30552289) [IF=6.5]

Application: WB    Species: pig    Sample: porcine intramuscular preadipocytes

Fig.?4.|Melatonin receptor (MT2) participates in lipolysis in precursor adipocytes. A: The mRNA levels of MT1 and MT2 mRNA were measured in melatonintreated mature adipocytes by RT-qPCR. B, C: Western blot analyses were performed to detect the changes of MT1 and MT2. D–F: RT-qPCR analysis of genes involved in lipolysis in mature adipocytes cultivated in GM, GM + melatonin, and GM + melatonin + 4-PPDOT medium for 48 h. Data are represented as the mean ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001; n = 3).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.