MTLR Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from HeLa cells, using MTLR antibody.

Western blot analysis of extracts from HeLa cells, using MTLR Antibody.

FIGURE 2 (A) Motilin (MTL) and MTL receptor (MTLR) fluorescence co-labeling in the spinal dorsal horn 2 h after acute incisional pain.

Product:	MTLR Antibody
Catalog:	DF4993
Description:	Rabbit polyclonal antibody to MTLR
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	45 KD; 45kD(Calculated).
Uniprot:	O43193
RRID:	AB_2837352

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

MTLR Antibody detects endogenous levels of total MTLR.

RRID:

AB_2837352
Cite Format: Affinity Biosciences Cat# DF4993, RRID:AB_2837352.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G-protein coupled receptor 38; GPR38; MLNR; Motilin receptor; MTLR; MTLR_HUMAN; MTLR1;

Immunogens

Immunogen:

Uniprot:

O43193(MTLR_HUMAN) >>Visit HPA database.

Gene(ID):

MLNR(2862)

Expression:

O43193 MTLR_HUMAN:

Expressed only in thyroid, stomach, and bone marrow.

Sequence:

O43193 MTLR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSPWNGSDGPEGAREPPWPALPPCDERRCSPFPLGALVPVTAVCLCLFVVGVSGNVVTVMLIGRYRDMRTTTNLYLGSMAVSDLLILLGLPFDLYRLWRSRPWVFGPLLCRLSLYVGEGCTYATLLHMTALSVERYLAICRPLRARVLVTRRRVRALIAVLWAVALLSAGPFLFLVGVEQDPGISVVPGLNGTARIASSPLASSPPLWLSRAPPPSPPSGPETAEAAALFSRECRPSPAQLGALRVMLWVTTAYFFLPFLCLSILYGLIGRELWSSRRPLRGPAASGRERGHRQTVRVLLVVVLAFIICWLPFHVGRIIYINTEDSRMMYFSQYFNIVALQLFYLSASINPILYNLISKKYRAAAFKLLLARKSRPRGFHRSRDTAGEVAGDTGGDTVGYTETSANVKTMG

PTMs - O43193 As Substrate

O43193

Site	PTM Type	Source
T71	Phosphorylation	Uniprot
T72	Phosphorylation	Uniprot
T73	Phosphorylation	Uniprot
S83	Phosphorylation	Uniprot
R136	Methylation	Uniprot
T296	Phosphorylation	Uniprot
T324	Phosphorylation	Uniprot
S327	Phosphorylation	Uniprot
T402	Phosphorylation	Uniprot
T404	Phosphorylation	Uniprot

Research Backgrounds

O43193_MTLR_HUMAN

Function:

Receptor for motilin.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed only in thyroid, stomach, and bone marrow.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Motilin and its receptor are expressed in the dorsal horn in a rat model of acute incisional pain: Intrathecal motilin injection alleviates pain behaviors. Frontiers in Neuroscience, 2023 (PubMed: 36816129) [IF=4.3]

Application: IF/ICC Species: Rat Sample: spinal cord

FIGURE 2 (A) Motilin (MTL) and MTL receptor (MTLR) fluorescence co-labeling in the spinal dorsal horn 2 h after acute incisional pain. (B) Co-labeling of MTLR and β-tubulin III fluorescence in the spinal dorsal horn. MTL (red), MTLR (green), β-tubulin III (violet), and DAPI (blue). Scale bar = 100 μm/20 μm.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MTLR Antibody - #DF4993