TAS2R10 Antibody - #DF5157

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Product: TAS2R10 Antibody
Catalog: DF5157
Description: Rabbit polyclonal antibody to TAS2R10
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 35 KD; 35kD(Calculated).
Uniprot: Q9NYW0
RRID: AB_2837347

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
TAS2R10 Antibody detects endogenous levels of total TAS2R10.
RRID:
AB_2837347
Cite Format: Affinity Biosciences Cat# DF5157, RRID:AB_2837347.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC126811; MGC126813; T2R10; T2R10_HUMAN; TAS2R10; Taste receptor family B member 2; Taste receptor type 2 member 10; Taste receptor, family B, member 2; Taste receptor, type 2, member 10; TRB2;

Immunogens

Immunogen:

A synthesized peptide derived from human TAS2R10, corresponding to a region within the internal amino acids.

Uniprot:

Q9NYW0(T2R10_HUMAN) >>Visit HPA database.

Gene(ID):
TAS2R10(50839)
Expression:
Q9NYW0 T2R10_HUMAN:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.

Sequence:
MLRVVEGIFIFVVVSESVFGVLGNGFIGLVNCIDCAKNKLSTIGFILTGLAISRIFLIWIIITDGFIQIFSPNIYASGNLIEYISYFWVIGNQSSMWFATSLSIFYFLKIANFSNYIFLWLKSRTNMVLPFMIVFLLISSLLNFAYIAKILNDYKTKNDTVWDLNMYKSEYFIKQILLNLGVIFFFTLSLITCIFLIISLWRHNRQMQSNVTGLRDSNTEAHVKAMKVLISFIILFILYFIGMAIEISCFTVRENKLLLMFGMTTTAIYPWGHSFILILGNSKLKQASLRVLQQLKCCEKRKNLRVT

PTMs - Q9NYW0 As Substrate

Site PTM Type Enzyme
T48 Phosphorylation
S53 Phosphorylation

Research Backgrounds

Function:

Gustducin-coupled strychnine receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.

Family&Domains:

Belongs to the G-protein coupled receptor T2R family.

Research Fields

· Organismal Systems > Sensory system > Taste transduction.

References

1). Biomimetic in vitro respiratory system using smooth muscle cells on ECIS chips for anti-asthma TCMs screening. Analytica Chimica Acta, 2021 (PubMed: 33926702) [IF=6.2]

Application: IF/ICC    Species: Mouse    Sample: ASM cells

Fig. 2. Identification of TAS2Rs in mouse ASM cells. (A) RT-qPCR of mTAS2Rs expression in mouse ASM cells, with b-actin as internal control. Results were normalized to expression of the Adora1. Data are collected from three independent experiments. (B) Immunostaining with DAPI (blue), anti-TAS2R10, and anti-TAS2R14 receptor antibody (red), scale bar ¼ 20 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

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