SMO Antibody | Affinity Biosciences

IHC
Cites

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DF5152 at 1/100 staining Rat skin tissue by IHC-P.

DF5152 at 1/100 staining Mouse spleen tissue by IHC-P.

DF5152 at 1/100 staining Human colorectal cancer by IHC-P.

DF5152 at 1/100 staining Human mammary cancer by IHC-P.

Fig.

FIGURE 5 |Curcumin suppresses the TGF-β1-activated Hedgehog signaling in HIBECs.

Fig.

Figure 2 Ubenimex blocked Hh signaling by targeting CD13 in liver cancer cell lines.

Product:	SMO Antibody
Catalog:	DF5152
Description:	Rabbit polyclonal antibody to SMO
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Chicken
Mol.Wt.:	86 KD; 86kD(Calculated).
Uniprot:	Q99835
RRID:	AB_2837342

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

SMO Antibody detects endogenous levels of total SMO.

RRID:

AB_2837342
Cite Format: Affinity Biosciences Cat# DF5152, RRID:AB_2837342.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Frizzled family member 11; FZD11; Gx; Gx protein; Protein Gx; SMO; SMO_HUMAN; SMOH; Smoothened frizzled family receptor; Smoothened homolog (Drosophila); Smoothened homolog; Smoothened homolog precursor (SMO) (Gx protein); Smoothened seven transmembrane spanning receptor;

Immunogens

Immunogen:

Uniprot:

Q99835(SMO_HUMAN) >>Visit HPA database.

Gene(ID):

SMO(6608)

Sequence:

Q99835 SMO_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAARPARGPELPLLGLLLLLLLGDPGRGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRLGEPTSNETLSCVIIFVIVYYALMAGVVWFVVLTYAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKQPIPDCEIKNRPSLLVEKINLFAMFGTGIAMSTWVWTKATLLIWRRTWCRLTGQSDDEPKRIKKSKMIAKAFSKRHELLQNPGQELSFSMHTVSHDGPVAGLAFDLNEPSADVSSAWAQHVTKMVARRGAILPQDISVTPVATPVPPEEQANLWLVEAEISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIPRLPQLPRQKCLVAAGAWGAGDSCRQGAWTLVSNPFCPEPSPPQDPFLPSAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Chicken

100

Rabbit

100

Xenopus

Sheep

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99835 As Substrate

Q99835

Site	PTM Type	Source
S32	Phosphorylation	Uniprot
S43	Phosphorylation	Uniprot
S47	Phosphorylation	Uniprot
S588	Phosphorylation	Uniprot
S590	Phosphorylation	Uniprot
T593	Phosphorylation	Uniprot
S595	Phosphorylation	Uniprot
S611	Phosphorylation	Uniprot
S615	Phosphorylation	Uniprot
S616	Phosphorylation	Uniprot
K674	Acetylation	Uniprot
S771	Phosphorylation	Uniprot
S785	Phosphorylation	Uniprot

Research Backgrounds

Q99835_SMO_HUMAN

Function:

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).

Subcellular Location:

Membrane>Multi-pass membrane protein. Cell projection>Cilium.

Subunit Structure:

Homodimer. Interacts with ARRB2 (By similarity). Interacts with KIF7. Interacts with BBS5 and BBS7; the interactions are indicative for the association of SMO with the BBsome complex to facilitate ciliary localization of SMO. Interacts with DLG5 and SDCBP (By similarity). Interacts with GAS8/DRC4.

Family&Domains:

Belongs to the G-protein coupled receptor Fz/Smo family.

Research Fields

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

References

1). GLI1 activation by non-classical pathway integrin αvβ3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death & Disease, 2019 (PubMed: 31366904) [IF=9.0]

Application: WB Species: mouse Sample: BGC823MCAs and SGC7901MCAs

Fig. S5 |a Western blottingting showing the most pronounced effect of Smo silencing in the shSmo #2 group in BGC823MCAs and SGC7901MCAs.

2). Effects of metformin on Sonic hedgehog subgroup medulloblastoma progression: In vitro and in vivo studies. Frontiers in Pharmacology, 2022 (PubMed: 36278239) [IF=5.6]

3). Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. DRUG DEVELOPMENT RESEARCH, 2019 (PubMed: 31403228) [IF=3.8]

Application: WB Species: human Sample: HIBECs

FIGURE 5 |Curcumin suppresses the TGF-β1-activated Hedgehog signaling in HIBECs. (a) Quantitative analysis of Shh, Gli1, Smo, Ptch-1, and Sufu protein expression.

4). Disruption of Gprasp2 down-regulates Hedgehog signaling and leads to apoptosis in auditory cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 34418635) [IF=3.1]

Application: WB Species: Mice Sample: HEI-OC1 cells

Fig. 4. Gprasp2-KO induced apoptosis in HEI-OC1 cells by inhibiting Hh signaling pathway. (A) Flow cytometry assay of Gprasp2-WT/KO HEI-OC1 cells with no PUR-treatments (WT, KO) or with PUR-treatments (WT þ PUR, KO þ PUR). (B) Statistics of apoptotic cells in WT/KO/WT þ PUR/KO þ PUR groups (n ¼ 3, *p < 0.05). (C) Western blot assay of the key molecules of apoptosis (Bcl2, Bax, Casepase3, cleaved-Casepase3) and Hh signaling pathway (Smo, Gli1, Gli2) in WT/KO/WT þ PUR/KO þ PUR groups. (D) Statistics of the key molecules of apoptosis (Bcl2, Bax, Casepase3, cleaved-Casepase3) and Hh signaling pathway (Smo, Gli1, Gli2) in WT/KO/WT þ PUR/KO þ PUR groups (n ¼ 3; *p < 0.05).

5). Dihydrotanshinone I Inhibits Pancreatic Cancer Progression via Hedgehog/ Gli Signal Pathway. Current cancer drug targets, 2023 (PubMed: 37018533) [IF=3.0]

6). Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells. Oncology Letters, 2019 (PubMed: 31452785) [IF=2.9]

7). Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer. Translational cancer research, 2023 (PubMed: 37969369) [IF=0.9]

Application: WB Species: Mouse Sample: liver cancer cell

Figure 2 Ubenimex blocked Hh signaling by targeting CD13 in liver cancer cell lines. (A-C) Western blot analysis of the protein expression levels of CD13, PTCH1, GLI1, SMO, and SHH in Huh7, HepG2, and PLC/PRF/5 cells treated with 0.05 or 0.1 mg/mL ubenimex. Densitometry analysis was used to normalize the expression levels of the proteins to those of β-actin; mean ± SD is indicative of three independent experiments (*, P

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SMO Antibody - #DF5152