HRH1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of extracts from COLO205 cells, using HRH1 antibody.

DF4980 at 1/100 staining Mouse colorectal tissue by IHC-P.

DF4980 at 1/100 staining Rat testis tissue by IHC-P.

DF4980 staining Hela cells by IF/ICC.

Product:	HRH1 Antibody
Catalog:	DF4980
Description:	Rabbit polyclonal antibody to HRH1
Application:	WB IHC IF/ICC
Reactivity:	Human
Mol.Wt.:	60 KD; 56kD(Calculated).
Uniprot:	P35367
RRID:	AB_2837329

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

HRH1 Antibody detects endogenous levels of total HRH1.

RRID:

AB_2837329
Cite Format: Affinity Biosciences Cat# DF4980, RRID:AB_2837329.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BPHS, mouse, homolog of; H1 R; H1R; HH1R; HisH1; Histamine H(1) receptor; Histamine H1 receptor; Histamine receptor H1; Histamine receptor subclass H1; Hrh1; HRH1_HUMAN;

Immunogens

Immunogen:

Uniprot:

P35367(HRH1_HUMAN) >>Visit HPA database.

Gene(ID):

HRH1(3269)

Sequence:

P35367 HRH1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLPNSSCLLEDKMCEGNKTTMASPQLMPLVVVLSTICLVTVGLNLLVLYAVRSERKLHTVGNLYIVSLSVADLIVGAVVMPMNILYLLMSKWSLGRPLCLFWLSMDYVASTASIFSVFILCIDRYRSVQQPLRYLKYRTKTRASATILGAWFLSFLWVIPILGWNHFMQQTSVRREDKCETDFYDVTWFKVMTAIINFYLPTLLMLWFYAKIYKAVRQHCQHRELINRSLPSFSEIKLRPENPKGDAKKPGKESPWEVLKRKPKDAGGGSVLKSPSQTPKEMKSPVVFSQEDDREVDKLYCFPLDIVHMQAAAEGSSRDYVAVNRSHGQLKTDEQGLNTHGASEISEDQMLGDSQSFSRTDSDTTTETAPGKGKLRSGSNTGLDYIKFTWKRLRSHSRQYVSGLHMNRERKAAKQLGFIMAAFILCWIPYFIFFMVIAFCKNCCNEHLHMFTIWLGYINSTLNPLIYPLCNENFKKTFKRILHIRS

PTMs - P35367 As Substrate

P35367

Site	PTM Type	Enzyme	Source
S68	Phosphorylation		Uniprot
S70	Phosphorylation		Uniprot
T140	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
T142	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
S230	Phosphorylation		Uniprot
S233	Phosphorylation		Uniprot
K238	Ubiquitination		Uniprot
S255	Phosphorylation		Uniprot
K261	Ubiquitination		Uniprot
S271	Phosphorylation		Uniprot
S275	Phosphorylation		Uniprot
T279	Phosphorylation		Uniprot
K284	Ubiquitination		Uniprot
S285	Phosphorylation		Uniprot
Y301	Phosphorylation		Uniprot
Y321	Phosphorylation		Uniprot
S378	Phosphorylation		Uniprot
S380	Phosphorylation		Uniprot
Y386	Phosphorylation		Uniprot
S396	Phosphorylation	Q9UQM7 (CAMK2A) , P17612 (PRKACA) , Q13976 (PRKG1) , P17252 (PRKCA)	Uniprot
S398	Phosphorylation	Q13976 (PRKG1) , P17252 (PRKCA) , Q9UQM7 (CAMK2A) , P25098 (GRK2) , P17612 (PRKACA)	Uniprot
Y401	Phosphorylation		Uniprot
T478	Phosphorylation	P17252 (PRKCA)	Uniprot

Research Backgrounds

P35367_HRH1_HUMAN

Function:

In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.

PTMs:

Phosphorylation at sites in the second and third cytoplasmic loops independently contribute to agonist-induced receptor downregulation.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)

References

1). Second-generation antipsychotics induce cardiotoxicity by disrupting spliceosome signaling: Implications from proteomic and transcriptomic analyses. Pharmacological Research, 2021 (PubMed: 34098070) [IF=9.3]

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HRH1 Antibody - #DF4980