Product: HRH1 Antibody
Catalog: DF4980
Description: Rabbit polyclonal antibody to HRH1
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 60 KD; 56kD(Calculated).
Uniprot: P35367
RRID: AB_2837329

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
HRH1 Antibody detects endogenous levels of total HRH1.
RRID:
AB_2837329
Cite Format: Affinity Biosciences Cat# DF4980, RRID:AB_2837329.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BPHS, mouse, homolog of; H1 R; H1R; HH1R; HisH1; Histamine H(1) receptor; Histamine H1 receptor; Histamine receptor H1; Histamine receptor subclass H1; Hrh1; HRH1_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSLPNSSCLLEDKMCEGNKTTMASPQLMPLVVVLSTICLVTVGLNLLVLYAVRSERKLHTVGNLYIVSLSVADLIVGAVVMPMNILYLLMSKWSLGRPLCLFWLSMDYVASTASIFSVFILCIDRYRSVQQPLRYLKYRTKTRASATILGAWFLSFLWVIPILGWNHFMQQTSVRREDKCETDFYDVTWFKVMTAIINFYLPTLLMLWFYAKIYKAVRQHCQHRELINRSLPSFSEIKLRPENPKGDAKKPGKESPWEVLKRKPKDAGGGSVLKSPSQTPKEMKSPVVFSQEDDREVDKLYCFPLDIVHMQAAAEGSSRDYVAVNRSHGQLKTDEQGLNTHGASEISEDQMLGDSQSFSRTDSDTTTETAPGKGKLRSGSNTGLDYIKFTWKRLRSHSRQYVSGLHMNRERKAAKQLGFIMAAFILCWIPYFIFFMVIAFCKNCCNEHLHMFTIWLGYINSTLNPLIYPLCNENFKKTFKRILHIRS

PTMs - P35367 As Substrate

Site PTM Type Enzyme
S68 Phosphorylation
S70 Phosphorylation
T140 Phosphorylation Q9UQM7 (CAMK2A)
T142 Phosphorylation Q9UQM7 (CAMK2A)
S230 Phosphorylation
S233 Phosphorylation
K238 Ubiquitination
S255 Phosphorylation
K261 Ubiquitination
S271 Phosphorylation
S275 Phosphorylation
T279 Phosphorylation
K284 Ubiquitination
S285 Phosphorylation
Y301 Phosphorylation
Y321 Phosphorylation
S378 Phosphorylation
S380 Phosphorylation
Y386 Phosphorylation
S396 Phosphorylation Q9UQM7 (CAMK2A) , P17612 (PRKACA) , Q13976 (PRKG1) , P17252 (PRKCA)
S398 Phosphorylation Q13976 (PRKG1) , P17252 (PRKCA) , Q9UQM7 (CAMK2A) , P25098 (GRK2) , P17612 (PRKACA)
Y401 Phosphorylation
T478 Phosphorylation P17252 (PRKCA)

Research Backgrounds

Function:

In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.

PTMs:

Phosphorylation at sites in the second and third cytoplasmic loops independently contribute to agonist-induced receptor downregulation.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

References

1). Second-generation antipsychotics induce cardiotoxicity by disrupting spliceosome signaling: Implications from proteomic and transcriptomic analyses. Pharmacological Research, 2021 (PubMed: 34098070) [IF=9.3]

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