GPR35 Antibody - #DF4973

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Product: GPR35 Antibody
Catalog: DF4973
Description: Rabbit polyclonal antibody to GPR35
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Bovine, Horse
Mol.Wt.: 34 KD; 34kD(Calculated).
Uniprot: Q9HC97
RRID: AB_2837326

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(83%), Horse(83%)
Clonality:
Polyclonal
Specificity:
GPR35 Antibody detects endogenous levels of total GPR35.
RRID:
AB_2837326
Cite Format: Affinity Biosciences Cat# DF4973, RRID:AB_2837326.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G protein coupled receptor 35; G protein coupled receptor 35; GPR 35; GPR35; KYNA receptor; Kynurenic acid receptor; Probable G protein coupled receptor 35; Probable G protein coupled receptor GPR35;

Immunogens

Immunogen:
Uniprot:

Q9HC97(GPR35_HUMAN) >>Visit HPA database.

Gene(ID):
GPR35(2859)
Expression:
Q9HC97 GPR35_HUMAN:

Predominantly expressed in immune and gastrointestinal tissues.

Sequence:
MNGTYNTCGSSDLTWPPAIKLGFYAYLGVLLVLGLLLNSLALWVFCCRMQQWTETRIYMTNLAVADLCLLCTLPFVLHSLRDTSDTPLCQLSQGIYLTNRYMSISLVTAIAVDRYVAVRHPLRARGLRSPRQAAAVCAVLWVLVIGSLVARWLLGIQEGGFCFRSTRHNFNSMAFPLLGFYLPLAVVVFCSLKVVTALAQRPPTDVGQAEATRKAARMVWANLLVFVVCFLPLHVGLTVRLAVGWNACALLETIRRALYITSKLSDANCCLDAICYYYMAKEFQEASALAVAPSAKAHKSQDSLCVTLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
83
Bovine
83
Pig
75
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HC97 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
S11 Phosphorylation
S129 Phosphorylation
S147 Phosphorylation
Y259 Phosphorylation
T261 Phosphorylation
S262 Phosphorylation

Research Backgrounds

Function:

Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Internalized to the cytoplasm after exposure to kynurenic acid.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in immune and gastrointestinal tissues.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Prognostic value of CXCL17 and CXCR8 expression in patients with colon cancer. Oncology Letters (PubMed: 32782587) [IF=2.9]

Application: IHC    Species: human    Sample: colon and tumor

Figure 1. |CXCL17 and CXCR8 expression levels in colon cancer and healthy tumour-adjacent samples. Representative micrographs of high expression (a and b)and low expression (c and d) of CXCL17 (A) and CXCR8 (B) in colon cancer samples and healthy tumour‑adjacent samples. Magnification, x200.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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