GPR35 Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/4

Western blot analysis of extracts from transfected 293 and HepG2, using GPR35 Antibody.

DF4973 at 1/100 staining human Prostate tissue sections by IHC-P.

DF4973 staining Hela cells by IF/ICC.

Figure 1.

Product:	GPR35 Antibody
Catalog:	DF4973
Description:	Rabbit polyclonal antibody to GPR35
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Bovine, Horse
Mol.Wt.:	34 KD; 34kD(Calculated).
Uniprot:	Q9HC97
RRID:	AB_2837326

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(83%), Horse(83%)

Clonality:

Polyclonal

Specificity:

GPR35 Antibody detects endogenous levels of total GPR35.

RRID:

AB_2837326
Cite Format: Affinity Biosciences Cat# DF4973, RRID:AB_2837326.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G protein coupled receptor 35; G protein coupled receptor 35; GPR 35; GPR35; KYNA receptor; Kynurenic acid receptor; Probable G protein coupled receptor 35; Probable G protein coupled receptor GPR35;

Immunogens

Immunogen:

Uniprot:

Q9HC97(GPR35_HUMAN) >>Visit HPA database.

Gene(ID):

GPR35(2859)

Expression:

Q9HC97 GPR35_HUMAN:

Predominantly expressed in immune and gastrointestinal tissues.

Sequence:

Q9HC97 GPR35_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNGTYNTCGSSDLTWPPAIKLGFYAYLGVLLVLGLLLNSLALWVFCCRMQQWTETRIYMTNLAVADLCLLCTLPFVLHSLRDTSDTPLCQLSQGIYLTNRYMSISLVTAIAVDRYVAVRHPLRARGLRSPRQAAAVCAVLWVLVIGSLVARWLLGIQEGGFCFRSTRHNFNSMAFPLLGFYLPLAVVVFCSLKVVTALAQRPPTDVGQAEATRKAARMVWANLLVFVVCFLPLHVGLTVRLAVGWNACALLETIRRALYITSKLSDANCCLDAICYYYMAKEFQEASALAVAPSAKAHKSQDSLCVTLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Pig

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HC97 As Substrate

Q9HC97

Site	PTM Type	Source
T4	Phosphorylation	Uniprot
S11	Phosphorylation	Uniprot
S129	Phosphorylation	Uniprot
S147	Phosphorylation	Uniprot
Y259	Phosphorylation	Uniprot
T261	Phosphorylation	Uniprot
S262	Phosphorylation	Uniprot

Research Backgrounds

Q9HC97_GPR35_HUMAN

Function:

Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Internalized to the cytoplasm after exposure to kynurenic acid.

Tissue Specificity:

Predominantly expressed in immune and gastrointestinal tissues.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Prognostic value of CXCL17 and CXCR8 expression in patients with colon cancer. Oncology Letters, 2020 (PubMed: 32782587) [IF=2.9]

Application: IHC Species: human Sample: colon and tumor

Figure 1. |CXCL17 and CXCR8 expression levels in colon cancer and healthy tumour-adjacent samples. Representative micrographs of high expression (a and b)and low expression (c and d) of CXCL17 (A) and CXCR8 (B) in colon cancer samples and healthy tumour‑adjacent samples. Magnification, x200.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

GPR35 Antibody - #DF4973