GPR133 Antibody | Affinity Biosciences

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IHC
IF/ICC

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Product:	GPR133 Antibody
Catalog:	DF4947
Description:	Rabbit polyclonal antibody to GPR133
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	96 KD; 97kD(Calculated).
Uniprot:	Q6QNK2
RRID:	AB_2837300

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

GPR133 Antibody detects endogenous levels of total GPR133.

RRID:

AB_2837300
Cite Format: Affinity Biosciences Cat# DF4947, RRID:AB_2837300.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp434B1272; FLJ16770; G protein coupled receptor 133; G protein coupled receptor PGR25; G-protein coupled receptor PGR25; GP133_HUMAN; Gpr133; MGC138512; MGC138514; PGR25; Probable G-protein coupled receptor 133;

Immunogens

Immunogen:

Uniprot:

Q6QNK2(AGRD1_HUMAN) >>Visit HPA database.

Gene(ID):

ADGRD1(283383)

Expression:

Q6QNK2 AGRD1_HUMAN:

Up-regulated in CD133(+) cell population of glioblastoma.

Sequence:

Q6QNK2 AGRD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEKLLRLCCWYSWLLLFYYNFQVRGVYSRSQDHPGFQVLASASHYWPLENVDGIHELQDTTGDIVEGKVNKGIYLKEEKGVTLLYYGRYNSSCISKPEQCGPEGVTFSFFWKTQGEQSRPIPSAYGGQVISNGFKVCSSGGRGSVELYTRDNSMTWEASFSPPGPYWTHVLFTWKSKEGLKVYVNGTLSTSDPSGKVSRDYGESNVNLVIGSEQDQAKCYENGAFDEFIIWERALTPDEIAMYFTAAIGKHALLSSTLPSLFMTSTASPVMPTDAYHPIITNLTEERKTFQSPGVILSYLQNVSLSLPSKSLSEQTALNLTKTFLKAVGEILLLPGWIALSEDSAVVLSLIDTIDTVMGHVSSNLHGSTPQVTVEGSSAMAEFSVAKILPKTVNSSHYRFPAHGQSFIQIPHEAFHRHAWSTVVGLLYHSMHYYLNNIWPAHTKIAEAMHHQDCLLFATSHLISLEVSPPPTLSQNLSGSPLITVHLKHRLTRKQHSEATNSSNRVFVYCAFLDFSSGEGVWSNHGCALTRGNLTYSVCRCTHLTNFAILMQVVPLELARGHQVALSSISYVGCSLSVLCLVATLVTFAVLSSVSTIRNQRYHIHANLSFAVLVAQVLLLISFRLEPGTTPCQVMAVLLHYFFLSAFAWMLVEGLHLYSMVIKVFGSEDSKHRYYYGMGWGFPLLICIISLSFAMDSYGTSNNCWLSLASGAIWAFVAPALFVIVVNIGILIAVTRVISQISADNYKIHGDPSAFKLTAKAVAVLLPILGTSWVFGVLAVNGCAVVFQYMFATLNSLQGLFIFLFHCLLNSEVRAAFKHKTKVWSLTSSSARTSNAKPFHSDLMNGTRPGMASTKLSPWDKSSHSAHRVDLSAV

PTMs - Q6QNK2 As Substrate

Q6QNK2

Site	PTM Type	Source
Y85	Phosphorylation	Uniprot
Y183	Phosphorylation	Uniprot
T187	Phosphorylation	Uniprot
S194	Phosphorylation	Uniprot
S255	Phosphorylation	Uniprot
S309	Phosphorylation	Uniprot
T827	Phosphorylation	Uniprot
T833	Phosphorylation	Uniprot
S841	Phosphorylation	Uniprot

Research Backgrounds

Q6QNK2_AGRD1_HUMAN

Function:

Orphan receptor. Signals via G(s)-alpha family of G-proteins. Has protumorigenic function especially in glioblastoma.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Up-regulated in CD133(+) cell population of glioblastoma.

Family&Domains:

A short peptide sequence (termed the Stachel sequence) in the C-terminal part of the extracellular domain (ECD) functions as a tethered agonist. Upon structural changes within the ECD, e.g. due to extracellular ligand binding or mechanical movements, this intramolecular agonist is exposed to the 7 transmembrane region, triggering G-protein activation.

The N-terminal domain and autocatalytic activity of ADGRD1 at the GPCR proteolysis site (GPS) are not required for G-protein coupling activity.

Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.

References

1). Targeting GPR133 via miR-106a-5p inhibits the proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) of glioma cells. The International journal of neuroscience, 2023 (PubMed: 37036013) [IF=1.7]

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GPR133 Antibody - #DF4947