FZD8 Antibody - #DF4931

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Product: FZD8 Antibody
Catalog: DF4931
Description: Rabbit polyclonal antibody to FZD8
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Dog, Xenopus
Mol.Wt.: 70 KD, 60 kDa; 73kD(Calculated).
Uniprot: Q9H461
RRID: AB_2837284

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FZD8 Antibody detects endogenous levels of total FZD8.
RRID:
AB_2837284
Cite Format: Affinity Biosciences Cat# DF4931, RRID:AB_2837284.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Frizzled 8 seven transmembrane spanning receptor; frizzled 8, seven transmembrane spanning receptor; Frizzled family receptor 8; frizzled homolog 8 (Drosophila); Frizzled homolog 8; Frizzled-8; FZ 8; Fz-8; FZ8; FZD 8; FZD8; FZD8_HUMAN; hFZ 8; hFz8; HGNC4046; Homolog of Drosophila Frizzled 8;

Immunogens

Immunogen:
Uniprot:

Q9H461(FZD8_HUMAN) >>Visit HPA database.

Gene(ID):
FZD8(8325)
Expression:
Q9H461 FZD8_HUMAN:

Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.

Sequence:
MEWGYLLEVTSLLAALALLQRSSGAAAASAKELACQEITVPLCKGIGYNYTYMPNQFNHDTQDEAGLEVHQFWPLVEIQCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERAKAGCAPLMRQYGFAWPDRMRCDRLPEQGNPDTLCMDYNRTDLTTAAPSPPRRLPPPPPGEQPPSGSGHGRPPGARPPHRGGGRGGGGGDAAAPPARGGGGGGKARPPGGGAAPCEPGCQCRAPMVSVSSERHPLYNRVKTGQIANCALPCHNPFFSQDERAFTVFWIGLWSVLCFVSTFATVSTFLIDMERFKYPERPIIFLSACYLFVSVGYLVRLVAGHEKVACSGGAPGAGGAGGAGGAAAGAGAAGAGAGGPGGRGEYEELGAVEQHVRYETTGPALCTVVFLLVYFFGMASSIWWVILSLTWFLAAGMKWGNEAIAGYSQYFHLAAWLVPSVKSIAVLALSSVDGDPVAGICYVGNQSLDNLRGFVLAPLVIYLFIGTMFLLAGFVSLFRIRSVIKQQDGPTKTHKLEKLMIRLGLFTVLYTVPAAVVVACLFYEQHNRPRWEATHNCPCLRDLQPDQARRPDYAVFMLKYFMCLVVGITSGVWVWSGKTLESWRSLCTRCCWASKGAAVGGGAGATAAGGGGGPGGGGGGGPGGGGGPGGGGGSLYSDVSTGLTWRSGTASSVSYPKQMPLSQV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Xenopus
100
Horse
0
Sheep
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H461 As Substrate

Site PTM Type Enzyme
Ubiquitination
Y376 Phosphorylation
K515 Ubiquitination
Y540 Phosphorylation
T541 Phosphorylation

Research Backgrounds

Function:

Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.

PTMs:

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Subcellular Location:

Membrane>Multi-pass membrane protein. Golgi apparatus. Cell membrane>Multi-pass membrane protein.
Note: Colocalizes with GOPC at the Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle.

Subunit Structure:

Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly with LRP5 or LRP6; the interaction is promoted by Wnt-binding and signaling and inhibited by DKK1. Interacts with GPOC, RSPO1 and RSPO3 (By similarity). Interacts with glypican GPC3.

Family&Domains:

The PDZ-binding motif mediates interaction with GOPC.

Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.

The FZ domain is involved in binding with Wnt ligands.

Belongs to the G-protein coupled receptor Fz/Smo family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

References

1). Pyrvinium pamoate can overcome artemisinin’s resistance in anaplastic thyroid cancer. BMC Complementary Medicine and Therapies, 2021 (PubMed: 34049534) [IF=3.9]

Application: WB    Species: Human    Sample: CAL-62 cells

Fig. 3 Activation of WNT signaling in 100 μM artemisinin (ART)-treated CAL-62 cells after 48 h. A Immunofluorescence labeling (× 40). Western blots of β-catenin in the nuclei. C Western blots of WNT7B, FZD8, and β-catenin. Gels/blots are cropped for the clear presentation of results. Samples derived from the same experiment and gels/blots were processed in parallel. Ratio, the ratio of integrated density (treatment and untreated cells); N, groups without ART treatment; ART, groups treated with 100 μM ART

Application: WB    Species: human    Sample: CAL-62 cells

Fig. 3 |Activation of WNT signaling in 100 μM artemisinin (ART)-treated CAL-62 cells after 48 h.C Western blots of WNT7B, FZD8, and β-catenin. Gels/blots are cropped for the clear presentation of results.Samples derived from the same experiment and gels/blots were processed in parallel. Ratio, the ratio of integrated density (treatment and untreated cells); N, groups without ART treatment; ART, groups treated with 100 μM ART

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