FZD6 Antibody | Affinity Biosciences

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Western blot analysis of extracts from LOVO cells using FZD6 antibody.

Product:	FZD6 Antibody
Catalog:	DF4930
Description:	Rabbit polyclonal antibody to FZD6
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Rabbit
Mol.Wt.:	79 KD; 79kD(Calculated).
Uniprot:	O60353
RRID:	AB_2837283

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Rabbit(83%)

Clonality:

Polyclonal

Specificity:

FZD6 Antibody detects endogenous levels of total FZD6.

RRID:

AB_2837283
Cite Format: Affinity Biosciences Cat# DF4930, RRID:AB_2837283.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Frizzled 6 seven transmembrane spanning receptor; Frizzled drosophila homolog of 6; Frizzled family receptor 6; Frizzled homolog 6 (Drosophila); Frizzled homolog 6; Frizzled-6; Frizzled6; Fz 6; Fz-6; Fz6; FZD 6; Fzd6; FZD6_HUMAN; Hfz 6; hFz6; NDNC10;

Immunogens

Immunogen:

Uniprot:

O60353(FZD6_HUMAN) >>Visit HPA database.

Gene(ID):

FZD6(8323)

Expression:

O60353 FZD6_HUMAN:

Detected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney.

Sequence:

O60353 FZD6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEMFTFLLTCIFLPLLRGHSLFTCEPITVPRCMKMAYNMTFFPNLMGHYDQSIAAVEMEHFLPLANLECSPNIETFLCKAFVPTCIEQIHVVPPCRKLCEKVYSDCKKLIDTFGIRWPEELECDRLQYCDETVPVTFDPHTEFLGPQKKTEQVQRDIGFWCPRHLKTSGGQGYKFLGIDQCAPPCPNMYFKSDELEFAKSFIGTVSIFCLCATLFTFLTFLIDVRRFRYPERPIIYYSVCYSIVSLMYFIGFLLGDSTACNKADEKLELGDTVVLGSQNKACTVLFMLLYFFTMAGTVWWVILTITWFLAAGRKWSCEAIEQKAVWFHAVAWGTPGFLTVMLLAMNKVEGDNISGVCFVGLYDLDASRYFVLLPLCLCVFVGLSLLLAGIISLNHVRQVIQHDGRNQEKLKKFMIRIGVFSGLYLVPLVTLLGCYVYEQVNRITWEITWVSDHCRQYHIPCPYQAKAKARPELALFMIKYLMTLIVGISAVFWVGSKKTCTEWAGFFKRNRKRDPISESRRVLQESCEFFLKHNSKVKHKKKHYKPSSHKLKVISKSMGTSTGATANHGTSAVAITSHDYLGQETLTEIQTSPETSMREVKADGASTPRLREQDCGEPASPAASISRLSGEQVDGKGQAGSVSESARSEGRISPKSDITDTGLAQSNNLQVPSSSEPSSLKGSTSLLVHPVSGVRKEQGGGCHSDT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Horse

Dog

Bovine

Sheep

Pig

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60353 As Substrate

O60353

Site	PTM Type	Source
	Ubiquitination	Uniprot
T5	Phosphorylation	Uniprot
K108	Ubiquitination	Uniprot
K148	Ubiquitination	Uniprot
T150	O-Glycosylation	Uniprot
K166	Ubiquitination	Uniprot
K191	Ubiquitination	Uniprot
S496	Phosphorylation	Uniprot
S519	Phosphorylation	Uniprot
S526	Phosphorylation	Uniprot
K532	Methylation	Uniprot
K532	Ubiquitination	Uniprot
K545	Ubiquitination	Uniprot
S606	Phosphorylation	Uniprot
T607	Phosphorylation	Uniprot
C615	S-Nitrosylation	Uniprot
S620	Phosphorylation	Uniprot
S624	Phosphorylation	Uniprot
S626	Phosphorylation	Uniprot
S629	Phosphorylation	Uniprot
K636	Ubiquitination	Uniprot
S641	Phosphorylation	Uniprot
S653	Phosphorylation	Uniprot
S656	Phosphorylation	Uniprot
T659	Phosphorylation	Uniprot
T661	Phosphorylation	Uniprot
K681	Ubiquitination	Uniprot

Research Backgrounds

O60353_FZD6_HUMAN

Function:

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear (By similarity).

PTMs:

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Subcellular Location:

Membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cell surface. Apical cell membrane>Multi-pass membrane protein. Cytoplasmic vesicle membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: Colocalizes with FZD3 at the apical face of cells (By similarity). Localizes to the endoplasmic reticulum membrane in the presence of LMBR1L (By similarity).

Tissue Specificity:

Subunit Structure:

Interacts with LMBR1L.

Family&Domains:

Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.

The FZ domain is involved in binding with Wnt ligands.

Belongs to the G-protein coupled receptor Fz/Smo family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

References

1). Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin‑induced in vitro ototoxicity in HEI‑OC1 auditory cells using gene expression profiling. Molecular Medicine Reports, 2020 (PubMed: 33174043) [IF=3.4]

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FZD6 Antibody - #DF4930