CCKAR Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293, using CCKAR Antibody.

Product:	CCKAR Antibody
Catalog:	DF4914
Description:	Rabbit polyclonal antibody to CCKAR
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Rabbit
Mol.Wt.:	44 KD; 48kD(Calculated).
Uniprot:	P32238
RRID:	AB_2837267

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(85%), Horse(85%), Rabbit(92%)

Clonality:

Polyclonal

Specificity:

CCKAR Antibody detects endogenous levels of total CCKAR.

RRID:

AB_2837267
Cite Format: Affinity Biosciences Cat# DF4914, RRID:AB_2837267.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCK A; CCK A receptor; CCK AR; CCK-A receptor; CCK1 R; CCK1-R; CCK1R; CCKA; CCKA receptor; CCKR Type A; CCKRA; Cholecystokinin 1 receptor; Cholecystokinin A receptor; Cholecystokinin receptor type A; Cholecystokinin type A receptor;

Immunogens

Immunogen:

Uniprot:

P32238(CCKAR_HUMAN) >>Visit HPA database.

Gene(ID):

CCKAR(886)

Sequence:

P32238 CCKAR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDVVDSLLVNGSNITPPCELGLENETLFCLDQPRPSKEWQPAVQILLYSLIFLLSVLGNTLVITVLIRNKRMRTVTNIFLLSLAVSDLMLCLFCMPFNLIPNLLKDFIFGSAVCKTTTYFMGTSVSVSTFNLVAISLERYGAICKPLQSRVWQTKSHALKVIAATWCLSFTIMTPYPIYSNLVPFTKNNNQTANMCRFLLPNDVMQQSWHTFLLLILFLIPGIVMMVAYGLISLELYQGIKFEASQKKSAKERKPSTTSSGKYEDSDGCYLQKTRPPRKLELRQLSTGSSSRANRIRSNSSAANLMAKKRVIRMLIVIVVLFFLCWMPIFSANAWRAYDTASAERRLSGTPISFILLLSYTSSCVNPIIYCFMNKRFRLGFMATFPCCPNPGPPGARGEVGEEEEGGTTGASLSRFSYSHMSASVPPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Horse

Bovine

Sheep

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32238 As Substrate

P32238

Site	PTM Type	Source
Y263	Phosphorylation	Uniprot
K308	Acetylation	Uniprot
K309	Acetylation	Uniprot

Research Backgrounds

P32238_CCKAR_HUMAN

Function:

Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Cholecystokinin Octapeptide Promotes ANP Secretion through Activation of NOX4–PGC-1α–PPARα/PPARγ Signaling in Isolated Beating Rat Atria. Oxidative Medicine and Cellular Longevity (PubMed: 35770043)

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CCKAR Antibody - #DF4914