PTGER2 Antibody - #DF4878

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Product: PTGER2 Antibody
Catalog: DF4878
Description: Rabbit polyclonal antibody to PTGER2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 40 KD; 40kD(Calculated).
Uniprot: P43116
RRID: AB_2837241

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Bovine(92%), Sheep(92%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
PTGER2 Antibody detects endogenous levels of total PTGER2.
RRID:
AB_2837241
Cite Format: Affinity Biosciences Cat# DF4878, RRID:AB_2837241.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EP2; PE2R2_HUMAN; PGE receptor EP2 subtype; PGE2 receptor EP2 subtype; Prostaglandin E receptor 2 EP2 subtype; Prostaglandin E receptor 2 subtype EP2 53kDa; Prostaglandin E receptor 2 subtype EP2; Prostaglandin E2 receptor; Prostaglandin E2 receptor EP2 subtype; Prostanoid EP2 receptor; Ptger2;

Immunogens

Immunogen:
Uniprot:

P43116(PE2R2_HUMAN) >>Visit HPA database.

Gene(ID):
PTGER2(5732)
Expression:
P43116 PE2R2_HUMAN:

Placenta and lung.

Sequence:
MGNASNDSQSEDCETRQWLPPGESPAISSVMFSAGVLGNLIALALLARRWRGDVGCSAGRRSSLSLFHVLVTELVFTDLLGTCLISPVVLASYARNQTLVALAPESRACTYFAFAMTFFSLATMLMLFAMALERYLSIGHPYFYQRRVSRSGGLAVLPVIYAVSLLFCSLPLLDYGQYVQYCPGTWCFIRHGRTAYLQLYATLLLLLIVSVLACNFSVILNLIRMHRRSRRSRCGPSLGSGRGGPGARRRGERVSMAEETDHLILLAIMTITFAVCSLPFTIFAYMNETSSRKEKWDLQALRFLSINSIIDPWVFAILRPPVLRLMRSVLCCRISLRTQDATQTSCSTQSDASKQADL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
92
Sheep
92
Dog
92
Pig
83
Horse
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P43116 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
T15 Phosphorylation
S33 Phosphorylation
S57 Phosphorylation
S62 Phosphorylation
T194 Phosphorylation
Y196 Phosphorylation
S240 Phosphorylation

Research Backgrounds

Function:

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Placenta and lung.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Renin secretion.

References

1). PGE2 inhibits neutrophil phagocytosis through the EP2R–cAMP–PTEN pathway. Immunity, Inflammation and Disease, 2022 (PubMed: 35759236) [IF=3.2]

Application: WB    Species: Human    Sample: HL-60 cells

Figure 2 PGE2 binding to EP2 receptors increases cAMP production in neutrophils. (A) HL-60 cells were pretreated with EP2R blocking antibody (1 μg/ml) or isotype control antibody (1 μg/ml) for 6 h, and then stimulated with PGE2 (1 μg/ml) for 12 h. The cells were collected and the cAMP content was determined by ELISA. *p 

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