OPN3 Antibody - #DF4877

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Product: OPN3 Antibody
Catalog: DF4877
Description: Rabbit polyclonal antibody to OPN3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 45 KD; 45kD(Calculated).
Uniprot: Q9H1Y3
RRID: AB_2837240

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(93%), Rabbit(93%), Dog(93%)
Clonality:
Polyclonal
Specificity:
OPN3 Antibody detects endogenous levels of total OPN3.
RRID:
AB_2837240
Cite Format: Affinity Biosciences Cat# DF4877, RRID:AB_2837240.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ECPN; Encephalopsin; ERO; NMO 1; NMO1; OPN 3; Opn3; OPN3_HUMAN; Opsin 3; Opsin-3; Opsin3; Panopsin;

Immunogens

Immunogen:
Uniprot:

Q9H1Y3(OPN3_HUMAN) >>Visit HPA database.

Gene(ID):
OPN3(23596)
Expression:
Q9H1Y3 OPN3_HUMAN:

Strongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord.

Sequence:
MYSGNRSGGHGYWDGGGAAGAEGPAPAGTLSPAPLFSPGTYERLALLLGSIGLLGVGNNLLVLVLYYKFQRLRTPTHLLLVNISLSDLLVSLFGVTFTFVSCLRNGWVWDTVGCVWDGFSGSLFGIVSIATLTVLAYERYIRVVHARVINFSWAWRAITYIWLYSLAWAGAPLLGWNRYILDVHGLGCTVDWKSKDANDSSFVLFLFLGCLVVPLGVIAHCYGHILYSIRMLRCVEDLQTIQVIKILKYEKKLAKMCFLMIFTFLVCWMPYIVICFLVVNGHGHLVTPTISIVSYLFAKSNTVYNPVIYVFMIRKFRRSLLQLLCLRLLRCQRPAKDLPAAGSEMQIRPIVMSQKDGDRPKKKVTFNSSSIIFIITSDESLSVDDSDKTNGSKVDVIQVRPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
93
Dog
93
Rabbit
93
Chicken
79
Zebrafish
64
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H1Y3 As Substrate

Site PTM Type Enzyme
Y140 Phosphorylation
K355 Ubiquitination
S380 Phosphorylation

Research Backgrounds

Function:

May play a role in encephalic photoreception.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Strongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

References

1). Opsin 3 mediates UVA-induced keratinocyte supranuclear melanin cap formation. Communications Biology, 2023 (PubMed: 36869204) [IF=5.9]

Application: WB    Species: Human    Sample: HEK

Fig. 3: UVA induces keratinocyte supranuclear melanin cap via OPN3.a OPN3 expression (green) colocalized with HEK marker Pan-CK (red) in skin explant with immunofluorescence staining, without UVA (top) or with UVA irradiation (bottom). Nuclei were counterstained with DAPI. Masson-Fontana (MF) staining demonstrated melanin cap formation. Images were analyzed by brightfield/fluorescence microscopy. Scale bar = 20 µm. b After siRNA inhibited OPN3 irradiated without or with UVA, WB was used to analyze changes in OPN3, Dync1i1, and DCTN1 expression levels in HEK. WB analyses were normalized using β-tubulin as a loading control, and the relative protein level was quantified using Quantity One software. n = 3 independent experiments. Statistical significance was determined by one-ANOVA with post-test. **P 

Application: IF/ICC    Species: Human    Sample: HEK

Fig. 2: UVA increases OPN3 expression in human epidermal keratinocytes.a, b RT-qPCR analysis of OPNs gene expression levels in HaCaT and HEK. OPNs mRNA levels were normalized to GAPDH levels (n = 3 independent experiments). Statistical significance was determined by one-ANOVA with post-test. ****P 

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