EDG6 Antibody - #DF4872

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Product: EDG6 Antibody
Catalog: DF4872
Description: Rabbit polyclonal antibody to EDG6
Application: WB IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 40 KD; 42kD(Calculated).
Uniprot: O95977
RRID: AB_2837235

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
EDG6 Antibody detects endogenous levels of total EDG6.
RRID:
AB_2837235
Cite Format: Affinity Biosciences Cat# DF4872, RRID:AB_2837235.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EDG6; Endothelial differentiation lysophosphatidic acid G protein coupled receptor 6; Endothelial differentiation G protein coupled receptor 6; Endothelial differentiation G-protein coupled receptor 6; Endothelial differentiation gene 6; LPC1; Lysophospholipid receptor C1; MGC129298; Putative G protein coupled receptor; S1P receptor 4; S1P receptor Edg-6; S1P receptor Edg6; S1P4; S1PR4; S1PR4_HUMAN; SLP4; Sphingosine 1 phosphate receptor 4; Sphingosine 1 phosphate receptor Edg6; Sphingosine 1-phosphate receptor 4; Sphingosine 1-phosphate receptor Edg-6;

Immunogens

Immunogen:

A synthesized peptide derived from human EDG6, corresponding to a region within C-terminal amino acids.

Uniprot:

O95977(S1PR4_HUMAN) >>Visit HPA database.

Gene(ID):
S1PR4(8698)
Expression:
O95977 S1PR4_HUMAN:

Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.

Sequence:
MNATGTPVAPESCQQLAAGGHSRLIVLHYNHSGRLAGRGGPEDGGLGALRGLSVAASCLVVLENLLVLAAITSHMRSRRWVYYCLVNITLSDLLTGAAYLANVLLSGARTFRLAPAQWFLREGLLFTALAASTFSLLFTAGERFATMVRPVAESGATKTSRVYGFIGLCWLLAALLGMLPLLGWNCLCAFDRCSSLLPLYSKRYILFCLVIFAGVLATIMGLYGAIFRLVQASGQKAPRPAARRKARRLLKTVLMILLAFLVCWGPLFGLLLADVFGSNLWAQEYLRGMDWILALAVLNSAVNPIIYSFRSREVCRAVLSFLCCGCLRLGMRGPGDCLARAVEAHSGASTTDSSLRPRDSFRGSRSLSFRMREPLSSISSVRSI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
75
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Identification of S1PR4 as an immune modulator for favorable prognosis in HNSCC through machine learning. iScience, 2023 (PubMed: 37680482) [IF=5.8]

Application: IF/ICC    Species: human    Sample:

Figure 7 Independent HNSCC Cohort Validation (A) Representative images of GZMB and IFNG IHC staining in the independent HNSCC cohort. Scale bar 1 represents 100 μm, and scale bar 2 represents 20 μm. (B) Representative images of S1PR4 and CD8 IF staining in the independent HNSCC cohort, showing the co-localization between S1PR4 and CD8. Scale bar represents 50 μm. (C) Heatmap showed the relative protein expression of S1PR4, CD8, GZMB, and IFNG in the independent HNSCC cohort. (D) Correlation heatmap of S1PR4, CD8, GZMB, and IFNG. At the protein level, S1PR4 was found to have the strongest correlation with CD8 (r = 0.82, p < 0.05). The S1PR4 was also found to be significantly correlated with both IFNG (r = 0.25, p < 0.05) and GZMB (r = 0.38, p < 0.05). (E) The Kaplan-Meier plot revealed that, at the protein level, the HNSCC patients with higher S1PR4 expression tended to have a better prognosis (p = 0.00041). (F) Representative images of the co-localization between S1PR4 and the cytotoxic markers of CD8+ T cells (IFNG and GZMB). Scale bar represents 20 μm. (G) Representative images of the co-localization between S1PR4 and CX3CR1. Scale bar represents 20 μm. HNSC, head and neck squamous cell carcinoma.
2). Xiaoyin Jiedu Granules may alleviate psoriasis-like skin diseases in mice by regulating sphingosine 1-phosphate receptor expression and reducing Th17 cells. Heliyon, 2023 (PubMed: 37636348) [IF=4.0]

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