EDG4 Antibody | Affinity Biosciences

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Western blot analysis of extracts from COLO205 cells, using EDG4 antibody.

Product:	EDG4 Antibody
Catalog:	DF4870
Description:	Rabbit polyclonal antibody to EDG4
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Sheep
Mol.Wt.:	39 KD; 39kD(Calculated).
Uniprot:	Q9HBW0
RRID:	AB_2837233

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Sheep(86%)

Clonality:

Polyclonal

Specificity:

EDG4 Antibody detects endogenous levels of total EDG4.

RRID:

AB_2837233
Cite Format: Affinity Biosciences Cat# DF4870, RRID:AB_2837233.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EDG 4; EDG-4; EDG4; Endothelial Cell Differentiation Gene 4; Endothelial differentiation lysophosphatidic acid G protein coupled receptor 4; FLJ93869; G protein coupled receptor; IPA2; LPA 2; LPA receptor 2; LPA receptor EDG4; LPA-2; LPA2; LPAR2; LPAR2_HUMAN; Lysophosphatidic acid receptor 2; Lysophosphatidic acid receptor Edg 4; Lysophosphatidic acid receptor Edg-4; Lysophosphatidic acid receptor EDG4;

Immunogens

Immunogen:

Uniprot:

Q9HBW0(LPAR2_HUMAN) >>Visit HPA database.

Gene(ID):

LPAR2(9170)

Expression:

Q9HBW0 LPAR2_HUMAN:

Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prostate. Little or no expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, ovary, small intestine, or colon.

Sequence:

Q9HBW0 LPAR2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVIMGQCYYNETIGFFYNNSGKELSSHWRPKDVVVVALGLTVSVLVLLTNLLVIAAIASNRRFHQPIYYLLGNLAAADLFAGVAYLFLMFHTGPRTARLSLEGWFLRQGLLDTSLTASVATLLAIAVERHRSVMAVQLHSRLPRGRVVMLIVGVWVAALGLGLLPAHSWHCLCALDRCSRMAPLLSRSYLAVWALSSLLVFLLMVAVYTRIFFYVRRRVQRMAEHVSCHPRYRETTLSLVKTVVIILGAFVVCWTPGQVVLLLDGLGCESCNVLAVEKYFLLLAEANSLVNAAVYSCRDAEMRRTFRRLLCCACLRQSTRESVHYTSSAQGGASTRIMLPENGHPLMDSTL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Sheep

Pig

Horse

Bovine

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HBW0 As Substrate

Q9HBW0

Site	PTM Type	Source
Y295	Phosphorylation	Uniprot
Y325	Phosphorylation	Uniprot
S334	Phosphorylation	Uniprot
S349	Phosphorylation	Uniprot
T350	Phosphorylation	Uniprot

Research Backgrounds

Q9HBW0_LPAR2_HUMAN

Function:

Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway. Stimulates phospholipase C (PLC) activity in a manner that is independent of RALA activation.

Subcellular Location:

Cell surface. Cell membrane>Multi-pass membrane protein.
Note: Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA colocalizes with RALA in the endocytic vesicles.

Tissue Specificity:

Subunit Structure:

Interacts with SLC9A3R2/NHERF2, MAGI3 and PLCB3. Interacts with RALA and GRK2.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

Restrictive clause

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For Research Use Only.
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EDG4 Antibody - #DF4870