Product: CLTR1 Antibody
Catalog: DF4865
Description: Rabbit polyclonal antibody to CLTR1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 38 KD; 39kD(Calculated).
Uniprot: Q9Y271
RRID: AB_2837228

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(83%)
Clonality:
Polyclonal
Specificity:
CLTR1 Antibody detects endogenous levels of total CLTR1.
RRID:
AB_2837228
Cite Format: Affinity Biosciences Cat# DF4865, RRID:AB_2837228.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CLTR1_HUMAN; CYSLT 1; CysLT(1); CYSLT1R; CYSLTR 1; CYSLTR; CysLTR vide supra; CysLTR1; Cysteinyl leukotriene D4 receptor; Cysteinyl leukotriene receptor 1; G-protein coupled receptor HG55; HG55; HMTMF81; LTD4 receptor; MGC46139;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y271 CLTR1_HUMAN:

Widely expressed, with highest levels in spleen and peripheral blood leukocytes. Lower expression in several tissues, such as lung (mostly in smooth muscle bundles and alveolar macrophages), placenta, small intestine, pancreas, colon and heart.

Sequence:
MDETGNLTVSSATCHDTIDDFRNQVYSTLYSMISVVGFFGNGFVLYVLIKTYHKKSAFQVYMINLAVADLLCVCTLPLRVVYYVHKGIWLFGDFLCRLSTYALYVNLYCSIFFMTAMSFFRCIAIVFPVQNINLVTQKKARFVCVGIWIFVILTSSPFLMAKPQKDEKNNTKCFEPPQDNQTKNHVLVLHYVSLFVGFIIPFVIIIVCYTMIILTLLKKSMKKNLSSHKKAIGMIMVVTAAFLVSFMPYHIQRTIHLHFLHNETKPCDSVLRMQKSVVITLSLAASNCCFDPLLYFFSGGNFRKRLSTFRKHSLSSVTYVPRKKASLPEKGEEICKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
83
Sheep
75
Horse
73
Pig
67
Bovine
67
Dog
58
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y271 As Substrate

Site PTM Type Enzyme
T8 Phosphorylation
S10 Phosphorylation
S307 Phosphorylation
T308 Phosphorylation
S313 Phosphorylation
S315 Phosphorylation
S316 Phosphorylation
S326 Phosphorylation

Research Backgrounds

Function:

Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with highest levels in spleen and peripheral blood leukocytes. Lower expression in several tissues, such as lung (mostly in smooth muscle bundles and alveolar macrophages), placenta, small intestine, pancreas, colon and heart.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Baicalin Ameliorates Radiation-Induced Lung Injury by Inhibiting the CysLTs/CysLT1 Signaling Pathway. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35783527)

Application: IHC    Species: Mice    Sample: lung tissue

Figure 4 BA reduced the expression level of CysLT1 in the lung tissue of mice (400-fold). IR: irradiation, BA: baicalin, and DXM: dexamethasone. Compared with the control group, ▲P < 0.05 and ▲▲P < 0.01; compared with the IR group, #P < 0.05 and ##P < 0.01. Data are expressed as the mean ± SD (n = 3) and were analyzed by one-way ANOVA followed by Tukey analysis.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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