BRS3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from HUVEC cells, using BRS3 antibody.

DF4855 at 1/100 staining human colorectal cancer by IHC-P.

Product:	BRS3 Antibody
Catalog:	DF4855
Description:	Rabbit polyclonal antibody to BRS3
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Rabbit
Mol.Wt.:	44 KD; 44kD(Calculated).
Uniprot:	P32247
RRID:	AB_2837219

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(83%), Rabbit(83%)

Clonality:

Polyclonal

Specificity:

BRS3 Antibody detects endogenous levels of total BRS3.

RRID:

AB_2837219
Cite Format: Affinity Biosciences Cat# DF4855, RRID:AB_2837219.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BB3; BLP receptor subtype 3; Bombesin like receptor 3; Bombesin receptor subtype 3; Bombesin receptor subtype-3; BRS 3; BRS-3; BRS3; BRS3_HUMAN;

Immunogens

Immunogen:

Uniprot:

P32247(BRS3_HUMAN) >>Visit HPA database.

Gene(ID):

BRS3(680)

Expression:

P32247 BRS3_HUMAN:

In germ cells in testis. Lung carcinoma cells.

Sequence:

P32247 BRS3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQRQPHSPNQTLISITNDTESSSSVVSNDNTNKGWSGDNSPGIEALCAIYITYAVIISVGILGNAILIKVFFKTKSMQTVPNIFITSLAFGDLLLLLTCVPVDATHYLAEGWLFGRIGCKVLSFIRLTSVGVSVFTLTILSADRYKAVVKPLERQPSNAILKTCVKAGCVWIVSMIFALPEAIFSNVYTFRDPNKNMTFESCTSYPVSKKLLQEIHSLLCFLVFYIIPLSIISVYYSLIARTLYKSTLNIPTEEQSHARKQIESRKRIARTVLVLVALFALCWLPNHLLYLYHSFTSQTYVDPSAMHFIFTIFSRVLAFSNSCVNPFALYWLSKSFQKHFKAQLFCCKAERPEPPVADTSLTTLAVMGTVPGTGSIQMSEISVTSFTGCSVKQAEDRF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32247 As Substrate

P32247

Site	PTM Type	Source
S175	Phosphorylation	Uniprot
S202	Phosphorylation	Uniprot
T204	Phosphorylation	Uniprot

Research Backgrounds

P32247_BRS3_HUMAN

Function:

Role in sperm cell division, maturation, or function. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

In germ cells in testis. Lung carcinoma cells.

Subunit Structure:

Interacts with C6orf89.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

BRS3 Antibody - #DF4855