AGTRL1 Antibody - #DF4853

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Product: AGTRL1 Antibody
Catalog: DF4853
Description: Rabbit polyclonal antibody to AGTRL1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog
Mol.Wt.: 43 KD; 43kD(Calculated).
Uniprot: P35414
RRID: AB_2837217

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Horse(92%), Rabbit(92%), Dog(83%)
Clonality:
Polyclonal
Specificity:
AGTRL1 Antibody detects endogenous levels of total AGTRL1.
RRID:
AB_2837217
Cite Format: Affinity Biosciences Cat# DF4853, RRID:AB_2837217.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AGTR L1; AGTRL 1; AGTRL1; Angiotensin II receptor like 1; Angiotensin receptor like 1; Angiotensin receptor-like 1; Apelin receptor; APJ (apelin) receptor; APJ; APJ receptor; APJ_HUMAN; APJR; APLNR; B78; FLJ90771; G protein coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; GPCR34; HG 11; HG11; HG11 orphan receptor; MGC45246; MSR; msr/apj;

Immunogens

Immunogen:
Uniprot:

P35414(APJ_HUMAN) >>Visit HPA database.

Gene(ID):
APLNR(187)
Expression:
P35414 APJ_HUMAN:

Expressed in heart, brain, kidney, stomach, spleen, thymus, lung, ovary, small intestine and colon, adipose tissues and pancreas (PubMed:8294032, PubMed:25639753). Expressed in glial cells, astrocytes and neuronal subpopulations (PubMed:8294032). Expressed in embryonic (ESCs) and induced (iPSCs) pluripotent stem cells (PubMed:25639753).

Sequence:
MEEGGDFDNYYGADNQSECEYTDWKSSGALIPAIYMLVFLLGTTGNGLVLWTVFRSSREKRRSADIFIASLAVADLTFVVTLPLWATYTYRDYDWPFGTFFCKLSSYLIFVNMYASVFCLTGLSFDRYLAIVRPVANARLRLRVSGAVATAVLWVLAALLAMPVMVLRTTGDLENTTKVQCYMDYSMVATVSSEWAWEVGLGVSSTTVGFVVPFTIMLTCYFFIAQTIAGHFRKERIEGLRKRRRLLSIIVVLVVTFALCWMPYHLVKTLYMLGSLLHWPCDFDLFLMNIFPYCTCISYVNSCLNPFLYAFFDPRFRQACTSMLCCGQSRCAGTSHSSSGEKSASYSSGHSQGPGPNMGKGGEQMHEKSIPYSQETLVVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Horse
92
Bovine
83
Dog
83
Pig
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35414 As Substrate

Site PTM Type Enzyme
S248 Phosphorylation
Y264 Phosphorylation
S348 Phosphorylation

Research Backgrounds

Function:

Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity. Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone (By similarity). May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis (By similarity). Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development (By similarity). Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure.

(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.

Subcellular Location:

Cell membrane.
Note: After exposure to apelin (APLN), internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane (By similarity). After exposure to apelin receptor early endogenous ligand (APELA), internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane (PubMed:25639753).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain, kidney, stomach, spleen, thymus, lung, ovary, small intestine and colon, adipose tissues and pancreas. Expressed in glial cells, astrocytes and neuronal subpopulations. Expressed in embryonic (ESCs) and induced (iPSCs) pluripotent stem cells.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

References

1). The extract of an herbal medicine Chebulae fructus inhibits hepatocellular carcinoma by suppressing the Apelin/APJ system. Frontiers in pharmacology, 2024 (PubMed: 38881868) [IF=5.6]

Application: WB    Species: Human    Sample: HCC cells

CFE inhibits the Apelin/APJ in HCC cells. (A) The transcriptome analysis was performed between ctrl- or CFE-treated PLC/PRF/5 cells. PCA analysis of ctrl and CFE groups. (B) The volcano diagram of ctrl and CFE groups. (C) The histogram of KEGG pathways. (D) Protein-protein interaction network of the differential genes related to signal transduction and cancer: overview. The dot size and number of connections indicate the importance of the protein in the network. (E) The heatmap of the top 10 important genes in the protein-protein interaction network. QRT-PCR assays were performed to detect the expression of (F) GNGT1, ADCY10, PIK3R2, (G) Aplein, and (H) APJ in PLC/PRF/5 cells after treated with or without CFE. (I) The level of Apelin in the serum of mice treated with vehicle control, CFE, or 5-FU using ELISA assays. (J) IHC staining of APJ in tumor tissues from different treatment groups. Scale bar: 50 μm. (K) Protein levels of APJ in PLC/PRF/5 and HepG2 cells are determined using Western blotting. GAPDH serves as a loading control. Representative bands are shown in the left panel, and relative band intensities are shown in the right panel. The data were presented as mean ± SD. *p < 0.05, **p < 0.01.

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