ADORA3 Antibody - #DF4851

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Product: ADORA3 Antibody
Catalog: DF4851
Description: Rabbit polyclonal antibody to ADORA3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 38 KD; 36kD(Calculated).
Uniprot: P0DMS8
RRID: AB_2837215

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
ADORA3 Antibody detects endogenous levels of total ADORA3.
RRID:
AB_2837215
Cite Format: Affinity Biosciences Cat# DF4851, RRID:AB_2837215.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A3AR; A3R; AA3R; AA3R_HUMAN; AD 026; AD026; Adenosine A3 receptor; Adenosine receptor A3; ADORA 3; ADORA3; ARA3; bA552M11.5; Gpcr2; RP11 552M11.7; TGPCR1;

Immunogens

Immunogen:
Uniprot:

P0DMS8(AA3R_HUMAN) >>Visit HPA database.

Gene(ID):
ADORA3(140)
Expression:
P0DMS8 AA3R_HUMAN:

Expressed in the lung and bone. Expressed at lower levels in osteosarcoma tissues (at protein level).

Sequence:
MPNNSTALSLANVTYITMEIFIGLCAIVGNVLVICVVKLNPSLQTTTFYFIVSLALADIAVGVLVMPLAIVVSLGITIHFYSCLFMTCLLLIFTHASIMSLLAIAVDRYLRVKLTVRYKRVTTHRRIWLALGLCWLVSFLVGLTPMFGWNMKLTSEYHRNVTFLSCQFVSVMRMDYMVYFSFLTWIFIPLVVMCAIYLDIFYIIRNKLSLNLSNSKETGAFYGREFKTAKSLFLVLFLFALSWLPLSIINCIIYFNGEVPQLVLYMGILLSHANSMMNPIVYAYKIKKFKETYLLILKACVVCHPSDSLDTSIEKNSE

PTMs - P0DMS8 As Substrate

Site PTM Type Enzyme
S213 Phosphorylation

Research Backgrounds

Function:

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the lung and bone. Expressed at lower levels in osteosarcoma tissues (at protein level).

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). The cumulative analgesic effect of repeated electroacupuncture is modulated by Adora3 in the SCDH of mice with neuropathic pain. Animal models and experimental medicine, 2024 (PubMed: 38992885) [IF=3.7]

Application: WB    Species: Mouse    Sample:

FIGURE 2 Multiple electroacupuncture sessions influence the Spinal Adora3 and related enzymes. (A–C) Relative mRNA expression levels of Adora3, CD73, and ADA. (D) Western blot showing protein expression. (E–G) Relative protein expression levels of Adora3, CD73, ADA, and GAPDH. (H) Pearson's correlation analysis of Adora3 and metabolizing enzymes with the nociceptive responses. On days 2EA, 4EA, and 7EA following surgery, samples were obtained from the lumber L4–L6 segments. The data are presented as the means ± SD. Statistical significance was considered at p 

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