Product: LBP Antibody
Catalog: DF4840
Description: Rabbit polyclonal antibody to LBP
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 53 KD; 53kD(Calculated).
Uniprot: P18428
RRID: AB_2837205

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
LBP Antibody detects endogenous levels of total LBP.
RRID:
AB_2837205
Cite Format: Affinity Biosciences Cat# DF4840, RRID:AB_2837205.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BPI fold containing family D, member 2; Bpifd2; LBP; LBP_HUMAN; LBP1; Lipopolysaccharide binding protein; Lipopolysaccharide-binding protein; LPS binding protein; Ly88; MGC22233; OTTHUMP00000030965; RP23-407H16.4;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P18428 LBP_HUMAN:

Detected in blood serum (at protein level).

Sequence:
MGALARALPSILLALLLTSTPEALGANPGLVARITDKGLQYAAQEGLLALQSELLRITLPDFTGDLRIPHVGRGRYEFHSLNIHSCELLHSALRPVPGQGLSLSISDSSIRVQGRWKVRKSFFKLQGSFDVSVKGISISVNLLLGSESSGRPTVTASSCSSDIADVEVDMSGDLGWLLNLFHNQIESKFQKVLESRICEMIQKSVSSDLQPYLQTLPVTTEIDSFADIDYSLVEAPRATAQMLEVMFKGEIFHRNHRSPVTLLAAVMSLPEEHNKMVYFAISDYVFNTASLVYHEEGYLNFSITDDMIPPDSNIRLTTKSFRPFVPRLARLYPNMNLELQGSVPSAPLLNFSPGNLSVDPYMEIDAFVLLPSSSKEPVFRLSVATNVSATLTFNTSKITGFLKPGKVKVELKESKVGLFNAELLEALLNYYILNTFYPKFNDKLAEGFPLPLLKRVQLYDLGLQIHKDFLFLGANVQYMRV

PTMs - P18428 As Substrate

Site PTM Type Enzyme
N394 N-Glycosylation
K454 Acetylation

Research Backgrounds

Function:

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.

Subcellular Location:

Secreted. Cytoplasmic granule membrane.
Note: Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in blood serum (at protein level).

Subunit Structure:

When bound to LPS, interacts (via C-terminus) with soluble and membrane-bound CD14.

Family&Domains:

Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

References

1). Lipopolysaccharide-binding protein expression is increased by stress and inhibits monoamine synthesis to promote depressive symptoms. Immunity, 2023 (PubMed: 36854305) [IF=32.4]

2). Ficus pandurata Hance Inhibits Ulcerative Colitis and Colitis-Associated Secondary Liver Damage of Mice by Enhancing Antioxidation Activity. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34966476)

Application: WB    Species: Mouse    Sample:

Figure 7 FPH alleviates secondary liver injury in colitis mice via suppressing inflammation and the LPS/LBP/CD14/TLR4/NF-κB signaling axis. Effect of FPH on the levels of TNFα (a), LPS (b), LBP (c), sCD14 (d), and IL-18 (e) in the liver tissues of UC mice determined by ELISA. The protein expressions of LBP, CD14, NLRP3, TNFα, TLR4, MyD88, NF-κB, and p-NF-κB in the liver tissues were evaluated by Western blot (f) and quantitative analysis for Western blot results by normalizing to β-actin (g). (h) The protein expression of NLRP3 in the liver tissues of UC mice was detected by IHC. Results were expressed as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01 vs. the control group; #p < 0.05, ##p < 0.01 vs. the model group (DSS only).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.