Carboxypeptidase A Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from K562 cells, using CARBOXYPEPTIDASE A1 antibody.

Product:	Carboxypeptidase A Antibody
Catalog:	DF4829
Description:	Rabbit polyclonal antibody to Carboxypeptidase A
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Dog
Mol.Wt.:	47 KD; 47kD(Calculated).
Uniprot:	P15085
RRID:	AB_2837194

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Dog(89%)

Clonality:

Polyclonal

Specificity:

Carboxypeptidase A Antibody detects endogenous levels of total Carboxypeptidase A.

RRID:

AB_2837194
Cite Format: Affinity Biosciences Cat# DF4829, RRID:AB_2837194.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

carboxypeptidase A1 (pancreatic); Carboxypeptidase A1; Carboxypeptidase A1 precursor; CBPA1_HUMAN; CPA; CPA1; Pancreatic carboxypeptidase A1; Procarboxypeptidase A1 pancreatic;

Immunogens

Immunogen:

Uniprot:

P15085(CBPA1_HUMAN) >>Visit HPA database.

Gene(ID):

CPA1(1357)

Sequence:

P15085 CBPA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRGLLVLSVLLGAVFGKEDFVGHQVLRISVADEAQVQKVKELEDLEHLQLDFWRGPAHPGSPIDVRVPFPSIQAVKIFLESHGISYETMIEDVQSLLDEEQEQMFAFRSRARSTDTFNYATYHTLEEIYDFLDLLVAENPHLVSKIQIGNTYEGRPIYVLKFSTGGSKRPAIWIDTGIHSREWVTQASGVWFAKKITQDYGQDAAFTAILDTLDIFLEIVTNPDGFAFTHSTNRMWRKTRSHTAGSLCIGVDPNRNWDAGFGLSGASSNPCSETYHGKFANSEVEVKSIVDFVKDHGNIKAFISIHSYSQLLMYPYGYKTEPVPDQDELDQLSKAAVTALASLYGTKFNYGSIIKAIYQASGSTIDWTYSQGIKYSFTFELRDTGRYGFLLPASQIIPTAKETWLALLTIMEHTLNHPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Chicken

Horse

Bovine

Sheep

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15085 As Substrate

P15085

Site	PTM Type	Source
Y152	Phosphorylation	Uniprot
Y158	Phosphorylation	Uniprot
S272	Phosphorylation	Uniprot
T274	Phosphorylation	Uniprot
T338	Phosphorylation	Uniprot
Y344	Phosphorylation	Uniprot
T346	Phosphorylation	Uniprot
S352	Phosphorylation	Uniprot

Research Backgrounds

P15085_CBPA1_HUMAN

Function:

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.

Subcellular Location:

Secreted.

Subunit Structure:

Monomer. May form a complex with proelastase 2.

Family&Domains:

Belongs to the peptidase M14 family.

Research Fields

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Protein digestion and absorption.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Carboxypeptidase A Antibody - #DF4829