Cathepsin D Antibody | Affinity Biosciences

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Western blot analysis of extracts from HeLa cells, using CTSD antibody.

Product:	Cathepsin D Antibody
Catalog:	DF4824
Description:	Rabbit polyclonal antibody to Cathepsin D
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	44 KD; 45kD(Calculated).
Uniprot:	P07339
RRID:	AB_2837189

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(86%), Bovine(88%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Cathepsin D Antibody detects endogenous levels of total Cathepsin D.

RRID:

AB_2837189
Cite Format: Affinity Biosciences Cat# DF4824, RRID:AB_2837189.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CatD; CATD_HUMAN; Cathepsin D; Cathepsin D heavy chain; CD; Ceroid lipofuscinosis neuronal 10; CLN10; CPSD; ctsd; Epididymis secretory sperm binding protein Li 130P; HEL S 130P; Lysosomal aspartyl peptidase; Lysosomal aspartyl protease; MGC2311;

Immunogens

Immunogen:

Uniprot:

P07339(CATD_HUMAN) >>Visit HPA database.

Gene(ID):

CTSD(1509)

Expression:

P07339 CATD_HUMAN:

Expressed in the aorta extracellular space (at protein level) (PubMed:20551380). Expressed in liver (at protein level) (PubMed:1426530).

Sequence:

P07339 CATD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQPSSLLPLALCLLAAPASALVRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Sheep

100

Dog

100

Bovine

Pig

Xenopus

Horse

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07339 As Substrate

P07339

Site	PTM Type	Source
K28	Ubiquitination	Uniprot
S37	Phosphorylation	Uniprot
S42	Phosphorylation	Uniprot
K49	Ubiquitination	Uniprot
K54	Ubiquitination	Uniprot
T63	O-Glycosylation	Uniprot
K127	Ubiquitination	Uniprot
N134	N-Glycosylation	Uniprot
K184	Ubiquitination	Uniprot
T250	Phosphorylation	Uniprot
S252	Phosphorylation	Uniprot
K253	Ubiquitination	Uniprot
N263	N-Glycosylation	Uniprot
K313	Ubiquitination	Uniprot
Y325	Phosphorylation	Uniprot
K331	Ubiquitination	Uniprot
S333	Phosphorylation	Uniprot
T334	Phosphorylation	Uniprot
T339	Phosphorylation	Uniprot
K341	Ubiquitination	Uniprot
Y347	Phosphorylation	Uniprot
K348	Ubiquitination	Uniprot
Y354	Phosphorylation	Uniprot
K357	Ubiquitination	Uniprot
Y394	Phosphorylation	Uniprot

Research Backgrounds

P07339_CATD_HUMAN

Function:

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

PTMs:

N- and O-glycosylated.

Undergoes proteolytic cleavage and activation by ADAM30.

As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.

Subcellular Location:

Lysosome. Melanosome. Secreted>Extracellular space.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).

Tissue Specificity:

Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).

Subunit Structure:

Consists of a light chain and a heavy chain. Interacts with ADAM30; this leads to activation of CTSD. Interacts with GRN; stabilizes CTSD; increases its proteolytic activity (By similarity).

Family&Domains:

Belongs to the peptidase A1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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Cathepsin D Antibody - #DF4824