PE2R4 Antibody - #DF4880

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Product: PE2R4 Antibody
Catalog: DF4880
Description: Rabbit polyclonal antibody to PE2R4
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 55 KD; 53kD(Calculated).
Uniprot: P35408
RRID: AB_2837172

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(80%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(90%), Dog(90%)
Clonality:
Polyclonal
Specificity:
PE2R4 Antibody detects endogenous levels of total PE2R4.
RRID:
AB_2837172
Cite Format: Affinity Biosciences Cat# DF4880, RRID:AB_2837172.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EP 4; EP4; EP4R; MGC126583; PE2R4_HUMAN; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostaglandin E receptor 4; Prostaglandin E receptor 4 subtype EP4; Prostaglandin E2 receptor; Prostaglandin E2 receptor EP4 subtype; Prostanoid EP4 receptor; PTGER 2; PTGER 4; PTGER2; PTGER4;

Immunogens

Immunogen:
Uniprot:

P35408(PE2R4_HUMAN) >>Visit HPA database.

Gene(ID):
PTGER4(5734)
Expression:
P35408 PE2R4_HUMAN:

High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.

Sequence:
MSTPGVNSSASLSPDRLNSPVTIPAVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTLLVSPVTIATYMKGQWPGGQPLCEYSTFILLFFSLSGLSIICAMSVERYLAINHAYFYSHYVDKRLAGLTLFAVYASNVLFCALPNMGLGSSRLQYPDTWCFIDWTTNVTAHAAYSYMYAGFSSFLILATVLCNVLVCGALLRMHRQFMRRTSLGTEQHHAAAAASVASRGHPAASPALPRLSDFRRRRSFRRIAGAEIQMVILLIATSLVVLICSIPLVVRVFVNQLYQPSLEREVSKNPDLQAIRIASVNPILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSRRERSGQHCSDSQRTSSAMSGHSRSFISRELKEISSTSQTLLPDLSLPDLSENGLGGRNLLPGVPGMGLAQEDTTSLRTLRISETSDSSQGQDSESVLLVDEAGGSGRAGPAPKGSSLQVTFPSETLNLSEKCI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Horse
90
Dog
90
Rabbit
90
Pig
80
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35408 As Substrate

Site PTM Type Enzyme
S222 Phosphorylation
S235 Phosphorylation
S245 Phosphorylation
S252 Phosphorylation
S259 Phosphorylation
K308 Ubiquitination
T335 Phosphorylation
S338 Phosphorylation
S354 Phosphorylation
S374 Phosphorylation
S377 Phosphorylation
S379 Phosphorylation
S382 Phosphorylation
S389 Phosphorylation
T394 Phosphorylation
S400 Phosphorylation
S430 Phosphorylation
T433 Phosphorylation
S460 Phosphorylation
K468 Ubiquitination
S484 Phosphorylation

Research Backgrounds

Function:

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

PTMs:

Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.

Subunit Structure:

Interacts with FEM1A.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Renin secretion.

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