PTGER3 Antibody | Affinity Biosciences

WB
IF/ICC

1/3

Western blot analysis of extracts from K562 cells, using PE2R3 antibody.

Product:	PTGER3 Antibody
Catalog:	DF4879
Description:	Rabbit polyclonal antibody to PTGER3
Application:	WB IHC IF/ICC
Reactivity:	Human
Mol.Wt.:	40 KD; 43kD(Calculated).
Uniprot:	P43115
RRID:	AB_2837171

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

PTGER3 Antibody detects endogenous levels of total PTGER3.

RRID:

AB_2837171
Cite Format: Affinity Biosciences Cat# DF4879, RRID:AB_2837171.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EP 3; EP3; EP3 I; EP3 II; EP3 III; EP3 IV; Ep3 prostanoid receptor; Ep3 receptor; EP3 subtype; EP3e; HGNC:9595; MGC141828; MGC141829; MGC27302; PE2R3_HUMAN; PGE receptor EP3 subtype; PGE2 R; PGE2 receptor EP3 subtype; PGE2-R; PGE2R; Pgerep3; Prostaglandin E receptor 3 (subtype EP3); Prostaglandin E receptor 3; Prostaglandin E receptor EP3 subtype 3 isoform; Prostaglandin E2 receptor; Prostaglandin e2 receptor ep3; Prostaglandin E2 receptor EP3 subtype; Prostaglandin receptor (PGE 2); Prostaglandin receptor 3, EP3, subtype; Prostanoid EP3 receptor; PTGER 3; Ptger3; Ptgerep3; Rep3; rEP3a; rEP3b;

Immunogens

Immunogen:

Uniprot:

P43115(PE2R3_HUMAN) >>Visit HPA database.

Gene(ID):

PTGER3(5733)

Expression:

P43115 PE2R3_HUMAN:

Detected in kidney (PubMed:8117308, PubMed:8135729). Expressed in small intestine, heart, pancreas, gastric fundic mucosa, mammary artery and pulmonary vessels.

Sequence:

P43115 PE2R3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKETRGYGGDAPFCTRLNHSYTGMWAPERSAEARGNLTRPPGSGEDCGSVSVAFPITMLLTGFVGNALAMLLVSRSYRRRESKRKKSFLLCIGWLALTDLVGQLLTTPVVIVVYLSKQRWEHIDPSGRLCTFFGLTMTVFGLSSLFIASAMAVERALAIRAPHWYASHMKTRATRAVLLGVWLAVLAFALLPVLGVGQYTVQWPGTWCFISTGRGGNGTSSSHNWGNLFFASAFAFLGLLALTVTFSCNLATIKALVSRCRAKATASQSSAQWGRITTETAIQLMGIMCVLSVCWSPLLIMMLKMIFNQTSVEHCKTHTEKQKECNFFLIAVRLASLNQILDPWVYLLLRKILLRKFCQIRYHTNNYASSSTSLPCQCSSTLMWSDHLER

PTMs - P43115 As Substrate

P43115

Site	PTM Type	Source
Y7	Phosphorylation	Uniprot
T15	Phosphorylation	Uniprot
T107	Phosphorylation	Uniprot

Research Backgrounds

P43115_PE2R3_HUMAN

Function:

Receptor for prostaglandin E2 (PGE2). The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G(i) proteins, and to an elevation of intracellular calcium. Required for normal development of fever in response to pyrinogens, including IL1B, prostaglandin E2 and bacterial lipopolysaccharide (LPS). Required for normal potentiation of platelet aggregation by prostaglandin E2, and thus plays a role in the regulation of blood coagulation. Required for increased HCO3(-) secretion in the duodenum in response to mucosal acidification, and thereby contributes to the protection of the mucosa against acid-induced ulceration. Not required for normal kidney function, normal urine volume and osmolality (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Detected in kidney. Expressed in small intestine, heart, pancreas, gastric fundic mucosa, mammary artery and pulmonary vessels.

Subunit Structure:

Interacts (via C-terminus) with MKLN1.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PTGER3 Antibody - #DF4879