CD168 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/4

Western blot analysis of extracts from Raji cells, using HMMR Antibody.

FIGURE 9 B4GALT4 knockdown regulated TGF‐beta1/TGF‐beta receptor‐1 signaling pathway and modified the expressions of PLK1 and RHAMM.

Figure 3 Knockdown of HMMR inhibited proliferation and invasion of HCC cells.

Product:	CD168 Antibody
Catalog:	DF4809
Description:	Rabbit polyclonal antibody to CD168
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Horse, Dog
Mol.Wt.:	85 KD; 84kD(Calculated).
Uniprot:	O75330
RRID:	AB_2837160

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(80%), Horse(80%), Dog(80%)

Clonality:

Polyclonal

Specificity:

CD168 Antibody detects endogenous levels of total CD168.

RRID:

AB_2837160
Cite Format: Affinity Biosciences Cat# DF4809, RRID:AB_2837160.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD168; CD168 antigen; HMMR; HMMR_HUMAN; Hyaluronan mediated motility receptor; Hyaluronan-mediated motility receptor (RHAMM); IHABP; Intracellular hyaluronic acid-binding protein; MGC119494; MGC119495; OTTHUMP00000196920; Receptor for hyaluronan-mediated motility; RHAMM;

Immunogens

Immunogen:

Uniprot:

O75330(HMMR_HUMAN) >>Visit HPA database.

Gene(ID):

HMMR(3161)

Expression:

O75330 HMMR_HUMAN:

Expressed in breast cancer cell lines and in normal breast tissue.

Sequence:

O75330 HMMR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSFPKAPLKRFNDPSGCAPSPGAYDVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDKDTTLPASARKVKSSESKESQKNDKDLKILEKEIRVLLQERGAQDRRIQDLETELEKMEARLNAALREKTSLSANNATLEKQLIELTRTNELLKSKFSENGNQKNLRILSLELMKLRNKRETKMRGMMAKQEGMEMKLQVTQRSLEESQGKIAQLEGKLVSIEKEKIDEKSETEKLLEYIEEISCASDQVEKYKLDIAQLEENLKEKNDEILSLKQSLEENIVILSKQVEDLNVKCQLLEKEKEDHVNRNREHNENLNAEMQNLKQKFILEQQEREKLQQKELQIDSLLQQEKELSSSLHQKLCSFQEEMVKEKNLFEEELKQTLDELDKLQQKEEQAERLVKQLEEEAKSRAEELKLLEEKLKGKEAELEKSSAAHTQATLLLQEKYDSMVQSLEDVTAQFESYKALTASEIEDLKLENSSLQEKAAKAGKNAEDVQHQILATESSNQEYVRMLLDLQTKSALKETEIKEITVSFLQKITDLQNQLKQQEEDFRKQLEDEEGRKAEKENTTAELTEEINKWRLLYEELYNKTKPFQLQLDAFEVEKQALLNEHGAAQEQLNKIRDSYAKLLGHQNLKQKIKHVVKLKDENSQLKSEVSKLRCQLAKKKQSETKLQEELNKVLGIKHFDPSKAFHHESKENFALKTPLKEGNTNCYRAPMECQESWK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Bovine

Sheep

Xenopus

Rabbit

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75330 As Substrate

O75330

Site	PTM Type	Source
S2	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
S41	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
T110	Phosphorylation	Uniprot
K114	Ubiquitination	Uniprot
K138	Ubiquitination	Uniprot
T198	Phosphorylation	Uniprot
S205	Phosphorylation	Uniprot
K215	Ubiquitination	Uniprot
K221	Ubiquitination	Uniprot
Y250	Phosphorylation	Uniprot
K251	Ubiquitination	Uniprot
K338	Ubiquitination	Uniprot
K350	Ubiquitination	Uniprot
S353	Phosphorylation	Uniprot
K419	Ubiquitination	Uniprot
K489	Ubiquitination	Uniprot
K604	Ubiquitination	Uniprot
K635	Ubiquitination	Uniprot
S656	Phosphorylation	Uniprot
K696	Ubiquitination	Uniprot
T703	Phosphorylation	Uniprot
Y713	Phosphorylation	Uniprot

Research Backgrounds

O75330_HMMR_HUMAN

Function:

Receptor for hyaluronic acid (HA) (By similarity). Involved in cell motility (By similarity). When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis (By similarity).

Subcellular Location:

Cell surface. Cytoplasm.

Tissue Specificity:

Expressed in breast cancer cell lines and in normal breast tissue.

Subunit Structure:

Subunit of the HARC complex. Interacts with ANKRD26.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

References

1). Coupled scRNA-seq and Bulk-seq reveal the role of HMMR in hepatocellular carcinoma. Frontiers in immunology, 2024 (PubMed: 38633247) [IF=7.3]

Application: WB Species: Human Sample: HCC cells

Figure 3 Knockdown of HMMR inhibited proliferation and invasion of HCC cells. (A) The knockdown efficiency of si-HMMRs have been testified by western blot in SMMC-7721, Huh7 and LM3. (B, C) The results of clone assays. (D, E) The results of Edu assays. (F, G) The results of wound healing assays.**P

2). The critical role of B4GALT4 in promoting microtubule spindle assembly in HCC through the regulation of PLK1 and RHAMM expression. JOURNAL OF CELLULAR PHYSIOLOGY, 2022 (PubMed: 34270095) [IF=5.6]

Application: WB Species: Human Sample: HepG2 cells

FIGURE 9 B4GALT4 knockdown regulated TGF‐beta1/TGF‐beta receptor‐1 signaling pathway and modified the expressions of PLK1 and RHAMM. (a) B4GALT4 knockdown downregulated the expression of lumican. (b) The expressions of B4GALT4, P‐Smad2, Smad2, PLK1, and RHAMM in HepG2 cells after different treatments as mentioned above were detected by Western blot analysis. (c) The comparison of the mRNA expression level of HMMR between tumor and normal samples. (d) Survival analysis of HMMR in HCC. (e) The protein interaction network diagram of RHAMM, PLK1, and other pathway‐related genes. (f) ChIP‐seq results showed that Smad2/3 and p300 binding regions are enriched around the promoter of PLK1. (g) Schematic illustration of the B4GALT4‐mediated regulatory network responsible for the microtubule spindle assembly in HCC (****p<0.0001). B4GALT, beta 1,4‐galactosyltransferase; HCC, hepatocellular carcinoma; mRNA, messenger RNA

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CD168 Antibody - #DF4809