ACTBL2 Antibody - #DF4799

(3)  
Product: ACTBL2 Antibody
Catalog: DF4799
Description: Rabbit polyclonal antibody to ACTBL2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Rabbit
Mol.Wt.: 40 KD; 42kD(Calculated).
Uniprot: Q562R1
RRID: AB_2837153

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
ACTBL2 Antibody detects endogenous levels of total ACTBL2.
RRID:
AB_2837153
Cite Format: Affinity Biosciences Cat# DF4799, RRID:AB_2837153.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACT; ACTBL_HUMAN; ACTBL2; actin like protein; actin, beta like 2; Beta-actin-like protein 2; DKFZp686D0972; kappa actin; Kappa-actin;

Immunogens

Immunogen:
Uniprot:

Q562R1(ACTBL_HUMAN) >>Visit HPA database.

Gene(ID):
ACTBL2(345651)
Sequence:
MTDNELSALVVDNGSGMCKAGFGGDDAPRAVFPSMIGRPRHQGVMVGMGQKDCYVGDEAQSKRGVLTLKYPIEHGVVTNWDDMEKIWYHTFYNELRVAPDEHPILLTEAPLNPKINREKMTQIMFEAFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHIVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYNFTTTAEREIVRDVKEKLCYVALDFEQEMVRAAASSSPERSYELPDGQVITIGNERFRCPEAIFQPSFLGIESSGIHETTFNSIMKCDVDIRKDLYANTVLSGGSTMYPGIADRMQKEIITLAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPPIVHRKCF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Rabbit
100
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q562R1 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
S7 Phosphorylation
S15 Phosphorylation
K51 Acetylation
K51 Methylation
K51 Ubiquitination
Y54 Phosphorylation
K62 Acetylation
K62 Ubiquitination
K69 Methylation
Y70 Phosphorylation
T107 Phosphorylation
T121 Phosphorylation
Y144 Phosphorylation
T161 Phosphorylation
Y170 Phosphorylation
T187 Phosphorylation
Y189 Phosphorylation
K192 Acetylation
K192 Methylation
K192 Ubiquitination
Y219 Phosphorylation
S240 Phosphorylation
Y241 Phosphorylation
T250 Phosphorylation
S266 Phosphorylation
S272 Phosphorylation
S273 Phosphorylation
K285 Ubiquitination
C286 S-Nitrosylation
K292 Sumoylation
K292 Ubiquitination
Y295 Phosphorylation
T298 Phosphorylation
S304 Phosphorylation
T305 Phosphorylation
Y307 Phosphorylation
R313 Methylation
T320 Phosphorylation
S324 Phosphorylation
K329 Acetylation
K329 Sumoylation
K329 Ubiquitination
K337 Sumoylation
S349 Phosphorylation

Research Backgrounds

Function:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

PTMs:

Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).

Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others (By similarity). Interacts with PFN1 and PFDN1.

Family&Domains:

Belongs to the actin family.

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