Product: TLK1 Antibody
Catalog: DF4792
Description: Rabbit polyclonal antibody to TLK1
Application: WB
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 87 KD; 87kD(Calculated).
Uniprot: Q9UKI8
RRID: AB_2837146

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
TLK1 Antibody detects endogenous levels of total TLK1.
RRID:
AB_2837146
Cite Format: Affinity Biosciences Cat# DF4792, RRID:AB_2837146.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PKU beta; PKU-beta; serine threonine protein kinase; Serine/threonine protein kinase tousled like 1; Serine/threonine-protein kinase tousled-like 1; SNARE protein kinase SNAK; Tlk1; TLK1_HUMAN; Tousled like kinase 1; Tousled-like kinase 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UKI8 TLK1_HUMAN:

Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.

Sequence:
MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKI8 As Substrate

Site PTM Type Enzyme
S22 Phosphorylation
S33 Phosphorylation
T38 Phosphorylation
S41 Phosphorylation
S54 Phosphorylation
T68 Phosphorylation
S72 Phosphorylation
S74 Phosphorylation
S77 Phosphorylation
T78 Phosphorylation
S80 Phosphorylation
S82 Phosphorylation
T89 Phosphorylation
S93 Phosphorylation
S94 Phosphorylation
S97 Phosphorylation
S100 Phosphorylation
S103 Phosphorylation
S105 Phosphorylation
S133 Phosphorylation
S134 Phosphorylation
S138 Phosphorylation
S158 Phosphorylation
S159 Phosphorylation
S170 Phosphorylation
S176 Phosphorylation
S188 Phosphorylation
T192 Phosphorylation
K234 Acetylation
T304 Phosphorylation
T313 Phosphorylation
S357 Phosphorylation
K412 Ubiquitination
S444 Phosphorylation
K485 Ubiquitination
K513 Ubiquitination
S563 Phosphorylation
S611 Phosphorylation
S679 Phosphorylation
T688 Phosphorylation
K700 Ubiquitination
S741 Phosphorylation
S743 Phosphorylation O14757 (CHEK1)
S744 Phosphorylation
S760 Phosphorylation

PTMs - Q9UKI8 As Enzyme

Substrate Site Source
Q99638 (RAD9A) S328 Uniprot
Q99638 (RAD9A) T355 Uniprot

Research Backgrounds

Function:

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.

Subunit Structure:

Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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