STK10 Antibody - #DF4783

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Product: STK10 Antibody
Catalog: DF4783
Description: Rabbit polyclonal antibody to STK10
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 120 KD; 112kD(Calculated).
Uniprot: O94804
RRID: AB_2837137

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
STK10 Antibody detects endogenous levels of total STK10.
RRID:
AB_2837137
Cite Format: Affinity Biosciences Cat# DF4783, RRID:AB_2837137.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

LOK; Lymphocyte oriented kinase; Lymphocyte-oriented kinase; PRO2729; Serine threonine protein kinase 10; Serine/threonine-protein kinase 10; STK10; STK10_HUMAN;

Immunogens

Immunogen:
Uniprot:

O94804(STK10_HUMAN) >>Visit HPA database.

Gene(ID):
STK10(6793)
Expression:
O94804 STK10_HUMAN:

Highly expressed in rapidly proliferating tissues (spleen, placenta, and peripheral blood leukocytes). Also expressed in brain, heart, skeletal muscle, colon, thymus, kidney, liver, small intestine and lung.

Sequence:
MAFANFRRILRLSTFEKRKSREYEHVRRDLDPNEVWEIVGELGDGAFGKVYKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVKLLGAYYHDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQMLEALNFLHSKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVMCETMKDTPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLEHPFVSSITSNKALRELVAEAKAEVMEEIEDGRDEGEEEDAVDAASTLENHTQNSSEVSPPSLNADKPLEESPSTPLAPSQSQDSVNEPCSQPSGDRSLQTTSPPVVAPGNENGLAVPVPLRKSRPVSMDARIQVAQEKQVAEQGGDLSPAANRSQKASQSRPNSSALETLGGEKLANGSLEPPAQAAPGPSKRDSDCSSLCTSESMDYGTNLSTDLSLNKEMGSLSIKDPKLYKKTLKRTRKFVVDGVEVSITTSKIISEDEKKDEEMRFLRRQELRELRLLQKEEHRNQTQLSNKHELQLEQMHKRFEQEINAKKKFFDTELENLERQQKQQVEKMEQDHAVRRREEARRIRLEQDRDYTRFQEQLKLMKKEVKNEVEKLPRQQRKESMKQKMEEHTQKKQLLDRDFVAKQKEDLELAMKRLTTDNRREICDKERECLMKKQELLRDREAALWEMEEHQLQERHQLVKQQLKDQYFLQRHELLRKHEKEREQMQRYNQRMIEQLKVRQQQEKARLPKIQRSEGKTRMAMYKKSLHINGGGSAAEQREKIKQFSQQEEKRQKSERLQQQQKHENQMRDMLAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQNLKEWRDKLRPRKKALEEDLNQKKREQEMFFKLSEEAECPNPSTPSKAAKFFPYSSADAS

PTMs - O94804 As Substrate

Site PTM Type Enzyme
R11 Methylation
S13 Phosphorylation
T14 Phosphorylation
S20 Phosphorylation
K56 Ubiquitination
K65 Ubiquitination
K151 Ubiquitination
K159 Ubiquitination
T166 Phosphorylation
S179 Phosphorylation
K181 Ubiquitination
K184 Ubiquitination
T185 Phosphorylation
S191 Phosphorylation
T195 Phosphorylation
Y197 Phosphorylation
T208 Phosphorylation
T259 Phosphorylation
K262 Ubiquitination
K277 Ubiquitination
K301 Ubiquitination
S387 Phosphorylation
T390 Phosphorylation
T391 Phosphorylation
S392 Phosphorylation
S413 Phosphorylation
S417 Phosphorylation
S438 Phosphorylation
S444 Phosphorylation
K446 Ubiquitination
S448 Phosphorylation
S450 Phosphorylation
S454 Phosphorylation
S455 Phosphorylation O94804 (STK10)
T459 Phosphorylation
S469 Phosphorylation
S485 Phosphorylation
S495 Phosphorylation
S514 Phosphorylation
T530 Phosphorylation
S545 Phosphorylation
S549 Phosphorylation
K621 Ubiquitination
S679 Phosphorylation
T688 Phosphorylation
K690 Acetylation
K701 Ubiquitination
K759 Ubiquitination
K796 Ubiquitination
K823 Ubiquitination
S824 Phosphorylation
K841 Ubiquitination
S844 Phosphorylation
K849 Ubiquitination
S951 Phosphorylation
T952 Phosphorylation P28482 (MAPK1)
S954 Phosphorylation
Y962 Phosphorylation
S968 Phosphorylation

PTMs - O94804 As Enzyme

Substrate Site Source
O94804 (STK10) S455 Uniprot
P15311 (EZR) T567 Uniprot
P26038 (MSN) T558 Uniprot
P35241 (RDX) T564 Uniprot
P53350 (PLK1) T210 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.

PTMs:

Autophosphorylates following homodimerization, leading to activation of the protein.

Subcellular Location:

Cell membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in rapidly proliferating tissues (spleen, placenta, and peripheral blood leukocytes). Also expressed in brain, heart, skeletal muscle, colon, thymus, kidney, liver, small intestine and lung.

Subunit Structure:

Homodimer; homodimerization is required for activation segment autophosphorylation.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Research Fields

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

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