BAG4 Antibody - #DF4769

(3)  
Product: BAG4 Antibody
Catalog: DF4769
Description: Rabbit polyclonal antibody to BAG4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 49 KD; 50kD(Calculated).
Uniprot: O95429
RRID: AB_2837123

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
BAG4 Antibody detects endogenous levels of total BAG4.
RRID:
AB_2837123
Cite Format: Affinity Biosciences Cat# DF4769, RRID:AB_2837123.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BAG 4; BAG family molecular chaperone regulator 4; BAG-4; BAG4; BAG4_HUMAN; Bcl 2 associated athanogene 4; Bcl-2-associated athanogene 4; BCL2 associated athanogene 4; Silencer of death domains; SODD;

Immunogens

Immunogen:
Uniprot:

O95429(BAG4_HUMAN) >>Visit HPA database.

Gene(ID):
BAG4(9530)
Expression:
O95429 BAG4_HUMAN:

Ubiquitous.

Sequence:
MSALRRSGYGPSDGPSYGRYYGPGGGDVPVHPPPPLYPLRPEPPQPPISWRVRGGGPAETTWLGEGGGGDGYYPSGGAWPEPGRAGGSHQEQPPYPSYNSNYWNSTARSRAPYPSTYPVRPELQGQSLNSYTNGAYGPTYPPGPGANTASYSGAYYAPGYTQTSYSTEVPSTYRSSGNSPTPVSRWIYPQQDCQTEAPPLRGQVPGYPPSQNPGMTLPHYPYGDGNRSVPQSGPTVRPQEDAWASPGAYGMGGRYPWPSSAPSAPPGNLYMTESTSPWPSSGSPQSPPSPPVQQPKDSSYPYSQSDQSMNRHNFPCSVHQYESSGTVNNDDSDLLDSQVQYSAEPQLYGNATSDHPNNQDQSSSLPEECVPSDESTPPSIKKIIHVLEKVQYLEQEVEEFVGKKTDKAYWLLEEMLTKELLELDSVETGGQDSVRQARKEAVCKIQAILEKLEKKGL

PTMs - O95429 As Substrate

Site PTM Type Enzyme
R5 Methylation
R6 Methylation
S7 Phosphorylation
Y9 Phosphorylation
Y17 Phosphorylation
R19 Methylation
R40 Methylation
S49 Phosphorylation
R51 Methylation
R53 Methylation
Y72 Phosphorylation
Y73 Phosphorylation
Y102 Phosphorylation
R108 Methylation
R110 Methylation
S175 Phosphorylation
S179 Phosphorylation
T181 Phosphorylation
S184 Phosphorylation
R185 Methylation
R201 Methylation
Y207 Phosphorylation
S210 Phosphorylation
T216 Phosphorylation
R237 Methylation
S245 Phosphorylation
Y249 Phosphorylation
S289 Phosphorylation
Y302 Phosphorylation
S303 Phosphorylation
S375 Phosphorylation
K403 Ubiquitination

Research Backgrounds

Function:

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling. Negative regulator of PRKN translocation to damaged mitochondria.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Binds to the ATPase domain of HSP/HSC70 chaperones. Binds to the death domain of TNFRSF1A in the absence of TNF and thereby prevents binding of adapter molecules such as TRADD or TRAF2. Binds to the death domain of TNFRSF12. Interacts with PRKN.

Research Fields

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

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