BAG3 Antibody | Affinity Biosciences

1/3

Western blot analysis of extracts from HepG2 cells(uv treatment), using BAG3 Antibody.

DF4768 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

DF4768 at 1/100 staining Mouse testis tissue by IHC-P.

Product:	BAG3 Antibody
Catalog:	DF4768
Description:	Rabbit polyclonal antibody to BAG3
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	62 KD; 62kD(Calculated).
Uniprot:	O95817
RRID:	AB_2837122

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

BAG3 Antibody detects endogenous levels of total BAG3.

RRID:

AB_2837122
Cite Format: Affinity Biosciences Cat# DF4768, RRID:AB_2837122.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BAG 3; BAG family molecular chaperone regulator 3; BAG-3; Bag3; BAG3_HUMAN; Bcl 2 binding protein; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; BCL2 associated athanogene 3; BCL2 binding athanogene 3; BIS; CAIR 1; Docking protein CAIR 1; Docking protein CAIR-1; MFM6;

Immunogens

Immunogen:

Uniprot:

O95817(BAG3_HUMAN) >>Visit HPA database.

Gene(ID):

BAG3(9531)

Sequence:

O95817 BAG3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSAATHSPMMQVASGNGDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPSEGPKETPSSANGPSREGSRLPPAREGHPVYPQLRPGYIPIPVLHEGAENRQVHPFHVYPQPGMQRFRTEAAAAAPQRSQSPLRGMPETTQPDKQCGQVAAAAAAQPPASHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRGYISIPVIHEQNVTRPAAQPSFHQAQKTHYPAQQGEYQTHQPVYHKIQGDDWEPRPLRAASPFRSSVQGASSREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRETAPVSQPENKPESKPGPVGPELPPGHIPIQVIRKEVDSKPVSQKPPPPSEKVEVKVPPAPVPCPPPSPGPSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95817 As Substrate

O95817

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
S7	Phosphorylation		Uniprot
K29	Acetylation		Uniprot
K60	Ubiquitination		Uniprot
Y86	Phosphorylation		Uniprot
R90	Methylation		Uniprot
Y93	Phosphorylation		Uniprot
R106	Methylation		Uniprot
Y114	Phosphorylation		Uniprot
R121	Methylation		Uniprot
R123	Methylation		Uniprot
R133	Methylation		Uniprot
S134	Phosphorylation		Uniprot
S136	Phosphorylation		Uniprot
R139	Methylation		Uniprot
K149	Ubiquitination		Uniprot
C151	S-Nitrosylation		Uniprot
S171	Phosphorylation		Uniprot
S173	Phosphorylation		Uniprot
S177	Phosphorylation		Uniprot
C179	S-Nitrosylation		Uniprot
S180	Phosphorylation		Uniprot
S187	Phosphorylation	Q05655 (PRKCD)	Uniprot
S190	Phosphorylation		Uniprot
S194	Phosphorylation		Uniprot
S195	Phosphorylation		Uniprot
S198	Phosphorylation		Uniprot
R203	Methylation		Uniprot
Y205	Phosphorylation		Uniprot
S207	Phosphorylation		Uniprot
R218	Methylation		Uniprot
S224	Phosphorylation		Uniprot
K230	Methylation		Uniprot
K230	Ubiquitination		Uniprot
Y233	Phosphorylation		Uniprot
Y240	Phosphorylation		Uniprot
T242	Phosphorylation		Uniprot
Y247	Phosphorylation		Uniprot
K249	Ubiquitination		Uniprot
R258	Methylation		Uniprot
R261	Methylation		Uniprot
S264	Phosphorylation		Uniprot
R267	Methylation		Uniprot
S268	Phosphorylation		Uniprot
S269	Phosphorylation		Uniprot
S274	Phosphorylation		Uniprot
S275	Phosphorylation		Uniprot
S279	Phosphorylation		Uniprot
S283	Phosphorylation		Uniprot
S284	Phosphorylation		Uniprot
T285	Phosphorylation	P42345 (MTOR)	Uniprot
S289	Phosphorylation	P42345 (MTOR)	Uniprot
S291	Phosphorylation		Uniprot
S315	Phosphorylation		Uniprot
K344	Ubiquitination		Uniprot
S377	Phosphorylation		Uniprot
S381	Phosphorylation		Uniprot
S385	Phosphorylation		Uniprot
S386	Phosphorylation		Uniprot
S389	Phosphorylation		Uniprot
T392	Phosphorylation		Uniprot
T406	Phosphorylation		Uniprot
K445	Acetylation		Uniprot
K445	Sumoylation		Uniprot
K445	Ubiquitination		Uniprot
K450	Ubiquitination		Uniprot
Y451	Phosphorylation		Uniprot
Y457	Phosphorylation		Uniprot
T459	Phosphorylation		Uniprot
K493	Ubiquitination		Uniprot
Y508	Phosphorylation		Uniprot

Research Backgrounds

O95817_BAG3_HUMAN

Function:

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Colocalizes with HSF1 to the nucleus upon heat stress (PubMed:26159920).

Subunit Structure:

Binds to the ATPase domain of HSP70/HSC70 chaperones. Interacts with BCL2. Interacts with phospholipase C-gamma proteins. Interacts with DNAJB6. Interacts (via BAG domain) with HSF1; this interaction occurs in normal and heat-shocked cells promoting HSF1 nucleocytoplasmic shuttling. Interacts with HSPA8 (via NBD). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD). Interacts (via WW domain 1) with SYNPO2 (via PPPY motif). Interacts with HSPB8.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

BAG3 Antibody - #DF4768