Product: PIK3CG Antibody
Catalog: DF4764
Description: Rabbit polyclonal antibody to PIK3CG
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Sheep, Xenopus
Mol.Wt.: 120 KD; 126kD(Calculated).
Uniprot: P48736
RRID: AB_2837118

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Sheep(82%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
PIK3CG Antibody detects endogenous levels of total PIK3CG.
RRID:
AB_2837118
Cite Format: Affinity Biosciences Cat# DF4764, RRID:AB_2837118.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1 phosphatidylinositol 3 kinase; 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; 5-bisphosphate 3-kinase catalytic subunit gamma isoform; p110 gamma; p110gamma; p120 PI3K; p120-PI3K; Phosphatidylinositol 3 kinase catalytic 110 kD gamma; Phosphatidylinositol 3 kinase gamma, p110 gamma; Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform; Phosphatidylinositol-4; Phosphoinositide 3 kinase catalytic gamma polypeptide; Phosphoinositide 3 kinase gamma catalytic subunit; PI3 kinase p110 subunit gamma; PI3-kinase subunit gamma; PI3CG; PI3K; PI3K-gamma; PI3Kgamma; PIK3; Pik3cg; PK3CG_HUMAN; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma; Serine/threonine protein kinase PIK3CG;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P48736 PK3CG_HUMAN:

Pancreas, skeletal muscle, liver and heart.

Sequence:
MELENYKQPVVLREDNCRRRRRMKPRSAAASLSSMELIPIEFVLPTSQRKCKSPETALLHVAGHGNVEQMKAQVWLRALETSVAADFYHRLGPHHFLLLYQKKGQWYEIYDKYQVVQTLDCLRYWKATHRSPGQIHLVQRHPPSEESQAFQRQLTALIGYDVTDVSNVHDDELEFTRRGLVTPRMAEVASRDPKLYAMHPWVTSKPLPEYLWKKIANNCIFIVIHRSTTSQTIKVSPDDTPGAILQSFFTKMAKKKSLMDIPESQSEQDFVLRVCGRDEYLVGETPIKNFQWVRHCLKNGEEIHVVLDTPPDPALDEVRKEEWPLVDDCTGVTGYHEQLTIHGKDHESVFTVSLWDCDRKFRVKIRGIDIPVLPRNTDLTVFVEANIQHGQQVLCQRRTSPKPFTEEVLWNVWLEFSIKIKDLPKGALLNLQIYCGKAPALSSKASAESPSSESKGKVQLLYYVNLLLIDHRFLLRRGEYVLHMWQISGKGEDQGSFNADKLTSATNPDKENSMSISILLDNYCHPIALPKHQPTPDPEGDRVRAEMPNQLRKQLEAIIATDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKWGQQEIVAKTYQLLARREVWDQSALDVGLTMQLLDCNFSDENVRAIAVQKLESLEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLRNKRIGHFLFWFLRSEIAQSRHYQQRFAVILEAYLRGCGTAMLHDFTQQVQVIEMLQKVTLDIKSLSAEKYDVSSQVISQLKQKLENLQNSQLPESFRVPYDPGLKAGALAIEKCKVMASKKKPLWLEFKCADPTALSNETIGIIFKHGDDLRQDMLILQILRIMESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAKIQQSTVGNTGAFKDEVLNHWLKEKSPTEEKFQAAVERFVYSCAGYCVATFVLGIGDRHNDNIMITETGNLFHIDFGHILGNYKSFLGINKERVPFVLTPDFLFVMGTSGKKTSPHFQKFQDICVKAYLALRHHTNLLIILFSMMLMTGMPQLTSKEDIEYIRDALTVGKNEEDAKKYFLDQIEVCRDKGWTVQFNWFLHLVLGIKQGEKHSA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Sheep
82
Xenopus
82
Horse
73
Dog
73
Rabbit
73
Zebrafish
55
Chicken
55
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48736 As Substrate

Site PTM Type Enzyme
K7 Ubiquitination
K103 Ubiquitination
S227 Phosphorylation
T228 Phosphorylation
T229 Phosphorylation
S230 Phosphorylation
T232 Phosphorylation
T250 Phosphorylation
K288 Ubiquitination
K444 Ubiquitination
K501 Ubiquitination
K553 Ubiquitination
S582 Phosphorylation P05771 (PRKCB)
K597 Ubiquitination
K606 Ubiquitination
T607 Phosphorylation
S620 Phosphorylation
T627 Phosphorylation
S636 Phosphorylation
S650 Phosphorylation
T746 Phosphorylation
K750 Ubiquitination
K756 Ubiquitination
Y757 Phosphorylation
K768 Ubiquitination
K770 Ubiquitination
K792 Ubiquitination
S853 Phosphorylation
K903 Ubiquitination
K912 Ubiquitination
S974 Phosphorylation
K1000 Acetylation
T1024 Phosphorylation P17612 (PRKACA)
S1101 Phosphorylation P48736 (PIK3CG)

PTMs - P48736 As Enzyme

Substrate Site Source
P07951-2 (TPM2) S61 Uniprot
P48736 (PIK3CG) S1101 Uniprot
Q01105-2 (SET) S9 Uniprot
Q01105-2 (SET) S93 Uniprot

Research Backgrounds

Function:

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contributes to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.

Subcellular Location:

Cytoplasm. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Pancreas, skeletal muscle, liver and heart.

Subunit Structure:

Heterodimer of a catalytic subunit PIK3CG and a PIK3R5 or PIK3R6 regulatory subunit. Interacts with GRK2 through the PIK helical domain. Interaction with GRK2 is required for targeting to agonist-occupied receptor. Interacts with PDE3B (By similarity). Interacts with TPM2. Interacts with EPHA8; regulates integrin-mediated cell adhesion to substrate. Interacts with HRAS; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6 (By similarity).

Family&Domains:

Belongs to the PI3/PI4-kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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