Cytochrome P450 8B1 Antibody - #DF4762

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Product: Cytochrome P450 8B1 Antibody
Catalog: DF4762
Description: Rabbit polyclonal antibody to Cytochrome P450 8B1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 58 KD; 58kD(Calculated).
Uniprot: Q9UNU6
RRID: AB_2837116

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Horse(85%), Sheep(92%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
Cytochrome P450 8B1 Antibody detects endogenous levels of total Cytochrome P450 8B1.
RRID:
AB_2837116
Cite Format: Affinity Biosciences Cat# DF4762, RRID:AB_2837116.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; CP8B; CP8B1_HUMAN; CYP12; CYP8B1; CYPVIIIB1; Cytochrome P450 8B1; Cytochrome P450 subfamily VIIIB (sterol 12 alpha hydroxylase) polypeptide 1; Cytochrome P450, family 8, subfamily B, polypeptide 1; Sterol 12-alpha-hydroxylase;

Immunogens

Immunogen:
Uniprot:

Q9UNU6(CP8B1_HUMAN) >>Visit HPA database.

Gene(ID):
CYP8B1(1582)
Expression:
Q9UNU6 CP8B1_HUMAN:

Liver.

Sequence:
MVLWGPVLGALLVVIAGYLCLPGMLRQRRPWEPPLDKGTVPWLGHAMAFRKNMFEFLKRMRTKHGDVFTVQLGGQYFTFVMDPLSFGSILKDTQRKLDFGQYAKKLVLKVFGYRSVQGDHEMIHSASTKHLRGDGLKDLNETMLDSLSFVMLTSKGWSLDASCWHEDSLFRFCYYILFTAGYLSLFGYTKDKEQDLLQAGELFMEFRKFDLLFPRFVYSLLWPREWLEVGRLQRLFHKMLSVSHSQEKEGISNWLGNMLQFLREQGVPSAMQDKFNFMMLWASQGNTGPTSFWALLYLLKHPEAIRAVREEATQVLGEARLETKQSFAFKLGALQHTPVLDSVVEETLRLRAAPTLLRLVHEDYTLKMSSGQEYLFRHGDILALFPYLSVHMDPDIHPEPTVFKYDRFLNPNGSRKVDFFKTGKKIHHYTMPWGSGVSICPGRFFALSEVKLFILLMVTHFDLELVDPDTPLPHVDPQRWGFGTMQPSHDVRFRYRLHPTE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Sheep
92
Dog
92
Rabbit
92
Horse
85
Chicken
69
Zebrafish
54
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UNU6 As Substrate

Site PTM Type Enzyme
T62 Phosphorylation
T69 Phosphorylation
Y76 Phosphorylation
T78 Phosphorylation
S127 Phosphorylation
S269 Phosphorylation
S326 Phosphorylation
T347 Phosphorylation
T355 Phosphorylation
S369 Phosphorylation
S370 Phosphorylation
S448 Phosphorylation

Research Backgrounds

Function:

Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Microsome membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Liver.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Antarctic krill peptide alleviated liver fibrosis via downregulating secondary bile acid activated NLRP3 signaling pathway. Food & Function, 2022 (PubMed: 35762853) [IF=6.1]
2). Obeticholic acid protects against lithocholic acid-induced exogenous cell apoptosis during cholestatic liver injury. Life sciences, 2024 (PubMed: 38104861) [IF=6.1]
3). An integrated strategy combining network toxicology and feature-based molecular networking for exploring hepatotoxic constituents and mechanism of Epimedii Folium-induced hepatotoxicity in vitro. Food and Chemical Toxicology, 2023 (PubMed: 37080529) [IF=4.3]

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