Cytochrome P450 3A7 Antibody | Affinity Biosciences

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Western blot analysis of extracts from LOVO cells, using Cytochrome P450 3A7 antibody.

Western blot analysis of extracts from Pc12, using Cytochrome P450 3A7 Antibody.

Western blot analysis of extracts from LOVO cells, using Cytochrome P450 3A7 Antibody.

Product:	Cytochrome P450 3A7 Antibody
Catalog:	DF4748
Description:	Rabbit polyclonal antibody to Cytochrome P450 3A7
Application:	WB
Reactivity:	Human, Rat
Mol.Wt.:	50 KD; 57kD(Calculated).
Uniprot:	P24462
RRID:	AB_2837102

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Polyclonal

Specificity:

Cytochrome P450 3A7 Antibody detects endogenous levels of total Cytochrome P450 3A7.

RRID:

AB_2837102
Cite Format: Affinity Biosciences Cat# DF4748, RRID:AB_2837102.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Aryl hydrocarbon hydroxylase; CP37; CP3A7_HUMAN; CYP3A7; CYPIIIA7; Cytochrome P450 3A7; Cytochrome P450, family 3, subfamily A, polypeptide 7; Cytochrome P450, subfamily IIIA, polypeptide 7; Cytochrome P450-HFLA; Flavoprotein linked monooxygenase; Microsomal monooxygenase; P 450(HFL33); P 450111A7; P450 HFLA; Xenobiotic monooxygenase;

Immunogens

Immunogen:

Uniprot:

P24462(CP3A7_HUMAN) >>Visit HPA database.

Gene(ID):

CYP3A7(100861540)

Expression:

P24462 CP3A7_HUMAN:

Expressed in fetal liver (at protein level).

Sequence:

P24462 CP3A7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDLIPNLAVETWLLLAVSLILLYLYGTRTHGLFKKLGIPGPTPLPFLGNALSFRKGYWTFDMECYKKYRKVWGIYDCQQPMLAITDPDMIKTVLVKECYSVFTNRRPFGPVGFMKNAISIAEDEEWKRIRSLLSPTFTSGKLKEMVPIIAQYGDVLVRNLRREAETGKPVTLKHVFGAYSMDVITSTSFGVSIDSLNNPQDPFVENTKKLLRFNPLDPFVLSIKVFPFLTPILEALNITVFPRKVISFLTKSVKQIKEGRLKETQKHRVDFLQLMIDSQNSKDSETHKALSDLELMAQSIIFIFAGYETTSSVLSFIIYELATHPDVQQKVQKEIDTVLPNKAPPTYDTVLQLEYLDMVVNETLRLFPVAMRLERVCKKDVEINGMFIPKGVVVMIPSYVLHHDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALVNMKLALVRVLQNFSFKPCKETQIPLKLRFGGLLLTEKPIVLKAESRDETVSGA

PTMs - P24462 As Substrate

P24462

Site	PTM Type	Source
Y75	Phosphorylation	Uniprot
S134	Phosphorylation	Uniprot
S284	Phosphorylation	Uniprot
T286	Phosphorylation	Uniprot
K406	Methylation	Uniprot
Y432	Phosphorylation	Uniprot
S437	Phosphorylation	Uniprot
T485	Phosphorylation	Uniprot

Research Backgrounds

P24462_CP3A7_HUMAN

Function:

A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins during embryogenesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA), a precursor in the biosynthesis of androgen and estrogen steroid hormones. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1), particularly D-ring hydroxylated estrone at the C16-alpha position. Mainly hydroxylates all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in atRA clearance during fetal development. Also involved in the oxidative metabolism of xenobiotics including anticonvulsants.

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein.

Tissue Specificity:

Expressed in fetal liver (at protein level).

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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Cytochrome P450 3A7 Antibody - #DF4748