PTGIS Antibody - #DF4745

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Product: PTGIS Antibody
Catalog: DF4745
Description: Rabbit polyclonal antibody to PTGIS
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 57 KD; 57kD(Calculated).
Uniprot: Q16647
RRID: AB_2837096

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(100%), Horse(91%), Sheep(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
PTGIS Antibody detects endogenous levels of total PTGIS.
RRID:
AB_2837096
Cite Format: Affinity Biosciences Cat# DF4745, RRID:AB_2837096.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CYP8; CYP8A1; Cytochrome P450, family 8, subfamily A, polypeptide 1; EC 5.3.99.4; MGC126858; MGC126860; OTTHUMP00000031777; PGIS; Prostacyclin synthase; Prostaglandin I2 (prostacyclin) synthase; Prostaglandin I2 synthase; PTGI; PTGIS; PTGIS_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q16647(PTGIS_HUMAN) >>Visit HPA database.

Gene(ID):
PTGIS(5740)
Expression:
Q16647 PTGIS_HUMAN:

Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate.

Sequence:
MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Horse
91
Dog
91
Pig
82
Zebrafish
64
Xenopus
56
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16647 As Substrate

Site PTM Type Enzyme
S52 Phosphorylation
S118 Phosphorylation
S200 Phosphorylation
Y398 Phosphorylation
T399 Phosphorylation

Research Backgrounds

Function:

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Vitamin D stimulates miR-26b-5p to inhibit placental COX-2 expression in preeclampsia. Scientific Reports, 2021 (PubMed: 34045549) [IF=4.6]

Application: IHC    Species: human    Sample: placental

Supplementary Figure 1|. Representative PGIS expression in placenta from normotensive pregnant women and women complicated with preeclampsia (PE). PGIS, prostacyclin synthase, Bar = 100 micron.
2). Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34007408)

Application: WB    Species: Rat    Sample: prostate cell

Figure 6 KLK1 could upregulate NO/cGMP signal and inhibit fibrosis in the prostate of aged rats. (a, b, e, and h) NO content, cGMP content, ADMA content, and cAMP content normalized to total protein concentration of rat prostate samples. (c, f, and i) Representative Western blot results of eNOS, DDAH2, COX-2, PTGIS, TGF-β1, RhoA, and ROCK1 in prostates of all three groups. (d, g, and j) The expression levels of above-mentioned proteins with β-actin as the loading control in all three groups. For each group, values are presented as the mean ± SD of 10 rats per group. ∗P < 0.05, ∗∗P < 0.01 (aTGR vs. aWTR). ##P < 0.01 (aWTR vs. yWTR).

Application: WB    Species: rat    Sample: prostate

Figure 6|: KLK1 could upregulate NO/cGMP signal and inhibit fibrosis in the prostate of aged rats.(c, f, and i) Representative Western blot results of eNOS, DDAH2, COX-2, PTGIS, TGF-β1, RhoA, and ROCK1 in prostates of all three groups.

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