RFC2 Antibody - #DF4735

(1)  
Product: RFC2 Antibody
Catalog: DF4735
Description: Rabbit polyclonal antibody to RFC2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 39 KD; 39kD(Calculated).
Uniprot: P35250
RRID: AB_2837086

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RFC2 Antibody detects endogenous levels of total RFC2.
RRID:
AB_2837086
Cite Format: Affinity Biosciences Cat# DF4735, RRID:AB_2837086.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A1 40 kDa subunit; A1; Activator 1 40 kDa subunit; Activator 1; Activator 1 subunit 2; MGC3665; OTTHUMP00000160645; OTTHUMP00000160646; Replication factor C (activator 1) 2 40kDa; Replication factor C (activator 1) 2; Replication factor C 2; Replication factor C 40 kDa subunit; Replication factor C subunit 2; RF C 40 kDa subunit; RF-C 40 kDa subunit; RFC 2; RFC 40; RFC 40 kDa subunit; RFC2; RFC2_HUMAN; RFC40;

Immunogens

Immunogen:
Uniprot:

P35250(RFC2_HUMAN) >>Visit HPA database.

Gene(ID):
RFC2(5982)
Sequence:
MEVEAVCGGAGEVEAQDSDPAPAFSKAPGSAGHYELPWVEKYRPVKLNEIVGNEDTVSRLEVFAREGNVPNIIIAGPPGTGKTTSILCLARALLGPALKDAMLELNASNDRGIDVVRNKIKMFAQQKVTLPKGRHKIIILDEADSMTDGAQQALRRTMEIYSKTTRFALACNASDKIIEPIQSRCAVLRYTKLTDAQILTRLMNVIEKERVPYTDDGLEAIIFTAQGDMRQALNNLQSTFSGFGFINSENVFKVCDEPHPLLVKEMIQHCVNANIDEAYKILAHLWHLGYSPEDIIGNIFRVCKTFQMAEYLKLEFIKEIGYTHMKIAEGVNSLLQMAGLLARLCQKTMAPVAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35250 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S18 Phosphorylation
S30 Phosphorylation
Y34 Phosphorylation
K41 Ubiquitination
K46 Ubiquitination
K82 Ubiquitination
K99 Ubiquitination
S108 Phosphorylation
R111 Methylation
K121 Ubiquitination
K127 Ubiquitination
T157 Phosphorylation
Y161 Phosphorylation
S162 Phosphorylation
K163 Acetylation
K163 Ubiquitination
T164 Phosphorylation
T165 Phosphorylation
S174 Phosphorylation
K176 Acetylation
K176 Ubiquitination
R184 Methylation
K192 Acetylation
K192 Ubiquitination
T200 Phosphorylation
K208 Ubiquitination
C255 S-Nitrosylation
K264 Ubiquitination
K304 Acetylation
K318 Ubiquitination
K347 Ubiquitination

Research Backgrounds

Function:

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA. RFC2 also interacts with PRKAR1A; the complex may be involved in cell survival. Interacts with DDX11.

Family&Domains:

Belongs to the activator 1 small subunits family.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.