Alkaline Phosphatase Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela, using ALPL Antibody.

DF4722 at 1/100 staining Mouse liver tissue by IHC-P.

Product:	Alkaline Phosphatase Antibody
Catalog:	DF4722
Description:	Rabbit polyclonal antibody to Alkaline Phosphatase
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	57 KD; 57kD(Calculated).
Uniprot:	P05186
RRID:	AB_2837073

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(100%), Dog(83%)

Clonality:

Polyclonal

Specificity:

ALPL Antibody detects endogenous levels of total ALPL.

RRID:

AB_2837073
Cite Format: Affinity Biosciences Cat# DF4722, RRID:AB_2837073.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; Alkaline phosphomonoesterase; Alpl; AP TNAP; AP-TNAP; APTNAP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT_HUMAN; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP;

Immunogens

Immunogen:

Uniprot:

P05186(PPBT_HUMAN) >>Visit HPA database.

Gene(ID):

ALPL(249)

Sequence:

P05186 PPBT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIGANLGHCAPASSAGSLAAGPLLLALALYPLSVLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05186 As Substrate

P05186

Site	PTM Type	Source
T48	Phosphorylation	Uniprot
S110	Phosphorylation	Uniprot
T113	Phosphorylation	Uniprot
S131	Phosphorylation	Uniprot
S160	Phosphorylation	Uniprot
N430	N-Glycosylation	Uniprot

Research Backgrounds

P05186_PPBT_HUMAN

Function:

This isozyme plays a key role in skeletal mineralization by regulating levels of diphosphate (PPi).

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Lipid-anchor.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the alkaline phosphatase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Thiamine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Multifunctional BPs/MT@PLGA-ALE Nanospheres for Treatment of Osteoporotic Fracture with Near-Infrared Irradiation. Advanced Functional Materials, 2023 [IF=19.0]

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Alkaline Phosphatase Antibody - #DF4722