IGF2BP2 Antibody - #DF4710

(1)   (2)  
Product: IGF2BP2 Antibody
Catalog: DF4710
Description: Rabbit polyclonal antibody to IGF2BP2
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 65 KD; 66kD(Calculated).
Uniprot: Q9Y6M1
RRID: AB_2837061

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
IGF2BP2 Antibody detects endogenous levels of total IGF2BP2.
RRID:
AB_2837061
Cite Format: Affinity Biosciences Cat# DF4710, RRID:AB_2837061.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hepatocellular carcinoma autoantigen p62; IF2B2_HUMAN; IGF 2 mRNA binding protein 2; IGF II mRNA binding protein 2; IGF-II mRNA-binding protein 2; IGF2 mRNA-binding protein 2; Igf2bp2; IMP 2; IMP-2; IMP2; Insulin like growth factor 2 mRNA binding protein 2; Insulin-like growth factor 2 mRNA-binding protein 2; OTTHUMP00000082606; OTTHUMP00000082607; OTTHUMP00000082608; p62; VICKZ 2; VICKZ family member 2; VICKZ2;

Immunogens

Immunogen:
Uniprot:

Q9Y6M1(IF2B2_HUMAN) >>Visit HPA database.

Gene(ID):
IGF2BP2(10644)
Expression:
Q9Y6M1 IF2B2_HUMAN:

Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.

Sequence:
MMNKLYIGNLSPAVTADDLRQLFGDRKLPLAGQVLLKSGYAFVDYPDQNWAIRAIETLSGKVELHGKIMEVDYSVSKKLRSRKIQIRNIPPHLQWEVLDGLLAQYGTVENVEQVNTDTETAVVNVTYATREEAKIAMEKLSGHQFENYSFKISYIPDEEVSSPSPPQRAQRGDHSSREQGHAPGGTSQARQIDFPLRILVPTQFVGAIIGKEGLTIKNITKQTQSRVDIHRKENSGAAEKPVTIHATPEGTSEACRMILEIMQKEADETKLAEEIPLKILAHNGLVGRLIGKEGRNLKKIEHETGTKITISSLQDLSIYNPERTITVKGTVEACASAEIEIMKKLREAFENDMLAVNQQANLIPGLNLSALGIFSTGLSVLSPPAGPRGAPPAAPYHPFTTHSGYFSSLYPHHQFGPFPHHHSYPEQEIVNLFIPTQAVGAIIGKKGAHIKQLARFAGASIKIAPAEGPDVSERMVIITGPPEAQFKAQGRIFGKLKEENFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPRDQTPDENEEVIVRIIGHFFASQTAQRKIREIVQQVKQQEQKYPQGVASQRSK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6M1 As Substrate

Site PTM Type Enzyme
Y6 Phosphorylation
S11 Phosphorylation
T15 Phosphorylation
Y40 Phosphorylation
Y45 Phosphorylation
Y73 Phosphorylation
K77 Methylation
K77 Ubiquitination
K78 Methylation
K139 Ubiquitination
Y148 Phosphorylation
S161 Phosphorylation
S162 Phosphorylation
S164 Phosphorylation
T186 Phosphorylation
S187 Phosphorylation
K221 Ubiquitination
T223 Phosphorylation
K232 Ubiquitination
K240 Ubiquitination
T269 Phosphorylation
K270 Ubiquitination
K278 Ubiquitination
K292 Ubiquitination
T324 Phosphorylation
T326 Phosphorylation
T330 Phosphorylation
S336 Phosphorylation
K451 Ubiquitination
S460 Phosphorylation
K462 Ubiquitination
K487 Methylation
K487 Ubiquitination
K497 Ubiquitination
K505 Ubiquitination
K509 Ubiquitination
S518 Phosphorylation
K530 Ubiquitination
T550 Phosphorylation
K583 Ubiquitination
K588 Ubiquitination

Research Backgrounds

Function:

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. In response to cellular stress, such as oxidative stress, recruited to stress granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.

Subunit Structure:

Can form homooligomers and heterooligomers with IGF2BP1 and IGF2BP3 in an RNA-dependent manner. Interacts with HNRPD and IGF2BP1. Interacts with ELAVL1, DHX9 and HNRNPU.

Family&Domains:

Domain KH3 and KH4 are the major RNA-binding modules, although KH1 and KH2 may also contribute. The contribution to RNA-binding of individual KH domains may be target-specific. KH1 and KH2, and possibly KH3 and KH4, promote the formation of higher ordered protein-RNA complexes, which may be essential for IGF2BP1 cytoplasmic retention. KH domains are required for RNA-dependent homo- and heterooligomerization and for localization to stress granules.

Belongs to the RRM IMP/VICKZ family.

References

1). Circular RNA 406961 interacts with ILF2 to regulate PM2.5-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways. ENVIRONMENT INTERNATIONAL, 2020 (PubMed: 32388272) [IF=11.8]

Application: WB    Species: Human    Sample: BEAS-2B cells

Fig. 4. The circ_406961 interacts with ILF2 protein. (A) Silver staining image of interaction proteins after circ_406961-pulldown experiment. NC indicates scramble control group; circ_406961 indicates protein group obtained by circ_406961; and Input indicates total protein group (up). Protein expression levels of ILF2, IGF2BP1, IGF2BP2, and IGF2BP3 in circ_406961-pulldown products (down). (B) ILF2, IGF2BP1, IGF2BP2, and IGF2BP3 protein expression levels in si-circ_406961 and PM2.5 co-treated BEAS-2B cells were tested by western blotting. (C) Gray values of ILF2, IGF2BP1, IGF2BP2, and IGF2BP3 protein expression levels. (D) ILF2, IGF2BP1, IGF2BP2, and IGF2BP3 protein expression levels in OE-circ_406961 and PM2.5 co-treated BEAS-2B cells were measured by western blotting. (E) Gray values of ILF2, IGF2BP1, IGF2BP2, and IGF2BP3 protein expression levels. (F) Bioinformatics analysis of interacting region and intensity between circ_406961 and ILF2. (G) Mass spectrogram of ILF2 protein. Data are shown as mean ± SD. *p < 0.05, **p < 0.01 vs control (0 μg/mL PM2.5). #p < 0.05, ##p < 0.01 vs NC (transiently transfected with scrambled sequence) or VEC (transiently transfected with blank plasmid vector).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.