DUSP10 Antibody - #DF4694

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Product: DUSP10 Antibody
Catalog: DF4694
Description: Rabbit polyclonal antibody to DUSP10
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 52 KD; 53kD(Calculated).
Uniprot: Q9Y6W6
RRID: AB_2837045

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
DUSP10 Antibody detects endogenous levels of total DUSP10.
RRID:
AB_2837045
Cite Format: Affinity Biosciences Cat# DF4694, RRID:AB_2837045.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dual specificity phosphatase 10; Dual specificity phosphatase MKP 5; Dual specificity phosphatase MKP5; Dual specificity protein phosphatase 10; DUS10_HUMAN; DUSP 10; DUSP10; DUSP10 protein; MAP kinase phosphatase 5; Mitogen activated protein kinase phosphatase 5; Mitogen-activated protein kinase phosphatase 5; MKP 5; MKP-5; MKP5; Serine/threonine specific protein phosphatase;

Immunogens

Immunogen:
Uniprot:

Q9Y6W6(DUS10_HUMAN) >>Visit HPA database.

Gene(ID):
DUSP10(11221)
Expression:
Q9Y6W6 DUS10_HUMAN:

Expressed in keratinocytes (at protein level) (PubMed:29043977). Detected in brain (PubMed:16806267).

Sequence:
MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSANPGSNSHPPVIATTVVSLKAANLTYMPSSSGSARSLNCGCSSASCCTVATYDKDNQAQTQAIAAGTTTTAIGTSTTCPANQMVNNNENTGSLSPSSGVGSPVSGTPKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPFMEYNKSHIQGAVHINCADKISRRRLQQGKITVLDLISCREGKDSFKRIFSKEIIVYDENTNEPSRVMPSQPLHIVLESLKREGKEPLVLKGGLSSFKQNHENLCDNSLQLQECREVGGGASAASSLLPQPIPTTPDIENAELTPILPFLFLGNEQDAQDLDTMQRLNIGYVINVTTHLPLYHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIVIAYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGVETVV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6W6 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S224 Phosphorylation P42345 (MTOR)
S230 Phosphorylation P42345 (MTOR)
Y236 Phosphorylation
T240 Phosphorylation
K380 Ubiquitination

Research Backgrounds

Function:

Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily. It preferably dephosphorylates p38.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in keratinocytes (at protein level). Detected in brain.

Subunit Structure:

Monomer. Interacts with MAPK14.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

References

1). DUSP10 is a novel immune-related biomarker connected with survival and cellular proliferation in lower-grade glioma. Aging (Albany NY), 2023 (PubMed: 37387540) [IF=3.9]

Application: WB    Species: Human    Sample: LGG tissues

Figure 7 In vitro experiments verification of DUSP10 in LGG. (A) Western blot analysis of DUSP10 expression in LGG tissues and corresponding para-carcinoma tissues. (B) Western blot and (C) qRT-PCR analysis of DUSP10 expression in NHA and LGG cell lines. (D) The cell viability of si-DUSP10-transfected and si-NC-transfected SW1088 cells by CCK-8 assays. (E, F) Effect of DUSP10 knockdown on colony formation was counted in SW1088 cells. (G, H) Representative images (G) and histogram analysis (H) of EdU assays after DUSP10 knockdown in SW1088 cells. (I, J) Cell cycle assays were implemented to evaluate the cell distribution of the SW1088 cell lines transfected with si-DUSP10 or si-NC lentiviruses.

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