DGKB Antibody | Affinity Biosciences

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Western blot analysis of extracts from HuvEC cells, using DGKB antibody.

Product:	DGKB Antibody
Catalog:	DF4693
Description:	Rabbit polyclonal antibody to DGKB
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Sheep, Rabbit, Chicken
Mol.Wt.:	90 KD; 91kD(Calculated).
Uniprot:	Q9Y6T7
RRID:	AB_2837044

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(89%), Sheep(100%), Rabbit(100%), Chicken(82%)

Clonality:

Polyclonal

Specificity:

DGKB Antibody detects endogenous levels of total DGKB.

RRID:

AB_2837044
Cite Format: Affinity Biosciences Cat# DF4693, RRID:AB_2837044.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

90 kDa diacylglycerol kinase; DAG kinase beta; DAGK2; DGK; DGK B; DGK beta; DGK-beta; Dgkb; DGKB_HUMAN; Diacylglycerol kinase beta (90kD); Diacylglycerol kinase beta 90kDa; Diacylglycerol kinase beta; Diglyceride kinase beta; KIAA0718;

Immunogens

Immunogen:

Uniprot:

Q9Y6T7(DGKB_HUMAN) >>Visit HPA database.

Gene(ID):

DGKB(1607)

Sequence:

Q9Y6T7 DGKB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPMVKSKPALLSGGLRMNKGAITPPRTTSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTLPTSGVSVPEERQSTVKKEKSGSQQPNKVIDKNKMQRANSVTVDGQGLQVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEKANVGKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKFEVIPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKRRSHRRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLVKRTRNRSKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Rabbit

100

Zebrafish

Chicken

Horse

Bovine

Dog

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6T7 As Substrate

Q9Y6T7

Site	PTM Type	Source
K113	Ubiquitination	Uniprot
S403	Phosphorylation	Uniprot
K408	Methylation	Uniprot
S420	Phosphorylation	Uniprot
Y586	Phosphorylation	Uniprot
S588	Phosphorylation	Uniprot
S594	Phosphorylation	Uniprot

Research Backgrounds

Q9Y6T7_DGKB_HUMAN

Function:

Exhibits high phosphorylation activity for long-chain diacylglycerols.

Subcellular Location:

Cytoplasm.

Family&Domains:

Belongs to the eukaryotic diacylglycerol kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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DGKB Antibody - #DF4693