GNE Antibody - #DF4690
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4690, RRID:AB_2837041.
Fold/Unfold
2310066H07Rik; Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase; DMRV; GLCNE_HUMAN; Glucosamine (UDP N acetyl) 2 epimerase/N acetylmannosamine kinase; GNE; IBM2; ManAc kinase; N acylmannosamine kinase; N-acetylmannosamine kinase; NM; RP23-209M8.6; Uae1; UDP GlcNAc 2 epimerase; UDP GlcNAc 2 epimerase/ManAc kinase; UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase; UDP-GlcNAc-2-epimerase; UDP-GlcNAc-2-epimerase/ManAc kinase; Uridine diphosphate N acetylglucosamine 2 epimerase; Uridine diphosphate-N-acetylglucosamine-2-epimerase;
Immunogens
Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon.
- Q9Y223 GLCNE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y223 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y22 | Phosphorylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K193 | Acetylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| K602 | Ubiquitination | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| K632 | Ubiquitination | Uniprot | |
| K637 | Ubiquitination | Uniprot |
Research Backgrounds
Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.
Phosphorylated by PKC.
Cytoplasm.
Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon.
Homodimer and homohexamer.
In the N-terminal section; belongs to the UDP-N-acetylglucosamine 2-epimerase family.
In the C-terminal section; belongs to the ROK (NagC/XylR) family.
Research Fields
· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.