TAOK3 Antibody - #DF4683

(3)  
Product: TAOK3 Antibody
Catalog: DF4683
Description: Rabbit polyclonal antibody to TAOK3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 110 KD; 105kD(Calculated).
Uniprot: Q9H2K8
RRID: AB_2837034

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(93%), Xenopus(93%)
Clonality:
Polyclonal
Specificity:
TAOK3 Antibody detects endogenous levels of total TAOK3.
RRID:
AB_2837034
Cite Format: Affinity Biosciences Cat# DF4683, RRID:AB_2837034.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CTCL tumor antigen HD CL 09; CTCL-associated antigen HD-CL-09; Cutaneous T cell lymphoma tumor antigen HD CL 09; Cutaneous T-cell lymphoma-associated antigen HD-CL-09; Dendritic cell derived protein kinase; Dendritic cell-derived protein kinase; DKFZp666H245; DPK; FLJ31808; hKFC A; hKFC-A; JIK; JNK/SAPK inhibitory kinase; JNK/SAPK-inhibitory kinase; Jun kinase inhibitory kinase; Jun kinase-inhibitory kinase; KDS; Kinase from chicken homolog A; MAP3K18; Serine kinase; Serine/threonine-protein kinase TAO3; STE20 like kinase; TAO kinase 3; TAOK3; TAOK3_HUMAN; Thousand and one amino acid protein 3;

Immunogens

Immunogen:
Uniprot:

Q9H2K8(TAOK3_HUMAN) >>Visit HPA database.

Gene(ID):
TAOK3(51347)
Expression:
Q9H2K8 TAOK3_HUMAN:

Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.

Sequence:
MRKGVLKDPEIADLFYKDDPEELFIGLHEIGHGSFGAVYFATNAHTSEVVAIKKMSYSGKQTHEKWQDILKEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALHGLAYLHSHALIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPTLQSNEWTDSFRRFVDYCLQKIPQERPTSAELLRHDFVRRDRPLRVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEDEEDSEHGTSLNREMDSLGSNHSIPSMSVSTGSQSSSVNSMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGDPRPEPRPTQSVQSQALHYRNRERFATIKSASLVTRQIHEHEQENELREQMSGYKRMRRQHQKQLIALENKLKAEMDEHRLKLQKEVETHANNSSIELEKLAKKQVAIIEKEAKVAAADEKKFQQQILAQQKKDLTTFLESQKKQYKICKEKIKEEMNEDHSTPKKEKQERISKHKENLQHTQAEEEAHLLTQQRLYYDKNCRFFKRKIMIKRHEVEQQNIREELNKKRTQKEMEHAMLIRHDESTRELEYRQLHTLQKLRMDLIRLQHQTELENQLEYNKRRERELHRKHVMELRQQPKNLKAMEMQIKKQFQDTCKVQTKQYKALKNHQLEVTPKNEHKTILKTLKDEQTRKLAILAEQYEQSINEMMASQALRLDEAQEAECQALRLQLQQEMELLNAYQSKIKMQTEAQHERELQKLEQRVSLRRAHLEQKIEEELAALQKERSERIKNLLERQEREIETFDMESLRMGFGNLVTLDFPKEDYR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
93
Chicken
93
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H2K8 As Substrate

Site PTM Type Enzyme
K7 Ubiquitination
K88 Ubiquitination
Y103 Phosphorylation
S177 Phosphorylation
T181 Phosphorylation Q9H2G2 (SLK)
Y183 Phosphorylation Q9H2G2 (SLK)
K260 Ubiquitination
Y305 Phosphorylation
K310 Ubiquitination
T316 Phosphorylation
S324 Phosphorylation Q13315 (ATM)
S331 Phosphorylation
T335 Phosphorylation
S336 Phosphorylation
S359 Phosphorylation Q13315 (ATM)
S421 Phosphorylation
Y429 Phosphorylation
T437 Phosphorylation
S440 Phosphorylation
S442 Phosphorylation
S462 Phosphorylation
S551 Phosphorylation Q13315 (ATM)
S572 Phosphorylation
T573 Phosphorylation
K642 Ubiquitination
T666 Phosphorylation
T745 Phosphorylation
S782 Phosphorylation
K817 Ubiquitination
K830 Acetylation
K855 Ubiquitination
Y897 Phosphorylation

Research Backgrounds

Function:

Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF).

PTMs:

Autophosphorylated. Phosphorylation at Ser-324 by ATM following DNA damage is required for activation of the p38/MAPK14 stress-activated MAPK cascade. Phosphorylated by LRRK2.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein.
Note: Also localized to the peripheral cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver.

Subunit Structure:

Self-associates. Interacts with ERN1 and TRAF2. Interaction with TRAF2 is facilitated under ER stress conditions, such as treatment with tunicamycin, and may promote TRAF2 phosphorylation.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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