SPON2 Antibody | Affinity Biosciences

WB
IHC
Cites

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SPON2 Antibody - Western blot analysis of extracts from 293 and mouse brain, using SPON2 Antibody.

SPON2 Antibody - DF4682 at 1/100 staining Mouse lung tissue by IHC-P.

SPON2 Antibody - DF4682 at 1/100 staining Rat lung tissue by IHC-P.

SPON2 Antibody - Figure 2.

Product:	SPON2 Antibody
Catalog:	DF4682
Description:	Rabbit polyclonal antibody to SPON2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Chicken, Xenopus
Mol.Wt.:	37 KD; 36kD(Calculated).
Uniprot:	Q9BUD6
RRID:	AB_2837033

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(90%), Horse(90%), Chicken(80%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

SPON2 Antibody detects endogenous levels of total SPON2.

RRID:

AB_2837033
Cite Format: Affinity Biosciences Cat# DF4682, RRID:AB_2837033.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Differentially expressed in cancerous and non-cancerous lung cells 1; DIL 1; DIL-1; DIL1; DKFZp686G21139; FLJ16313; FLJ22401; FLJ34460; M spondin; Mindin; ORF Names:;antibody;SPON 2; Spon2; SPON2_HUMAN; Spondin 2; Spondin 2 extracellular matrix protein; Spondin-2;

Immunogens

Immunogen:

Uniprot:

Q9BUD6(SPON2_HUMAN) >>Visit HPA database.

Gene(ID):

SPON2(10417)

Expression:

Q9BUD6 SPON2_HUMAN:

Expressed in normal lung tissue but not in lung carcinoma cell lines.

Sequence:

Q9BUD6 SPON2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENPSPAAALGKALCALLLATLGAAGQPLGGESICSARALAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHEVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Xenopus

Chicken

Sheep

Dog

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BUD6 As Substrate

Q9BUD6

Site	PTM Type	Source
Y89	Phosphorylation	Uniprot
S147	Phosphorylation	Uniprot
S260	Phosphorylation	Uniprot
W283	C-Glycosylation	Uniprot
Y304	Phosphorylation	Uniprot

Research Backgrounds

Q9BUD6_SPON2_HUMAN

Function:

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity:

Expressed in normal lung tissue but not in lung carcinoma cell lines.

Subunit Structure:

Monomer. Interacts with integrin.

References

1). Upregulation of Spondin-2 protein expression correlates with poor prognosis in hepatocellular carcinoma. The Journal of international medical research, 2019 (PubMed: 30318967) [IF=1.4]

Application: IHC Species: Human Sample: HCC and liver tissue

Figure 2. Expression of SPON2 in HCC and normal liver tissue. (a) qRT-PCR analysis of SPON2 mRNA expression in 20 paired HCC and matched non-cancerous liver tissues. (b) TMA-IHC analysis of SPON2 protein expression in 107 paired HCC and matched non-cancerous liver tissues.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

SPON2 Antibody - #DF4682