ARX Antibody - #DF4674

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Product: ARX Antibody
Catalog: DF4674
Description: Rabbit polyclonal antibody to ARX
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Sheep, Rabbit, Dog
Mol.Wt.: 55 KD; 58kD(Calculated).
Uniprot: Q96QS3
RRID: AB_2837025

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Sheep(100%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ARX Antibody detects endogenous levels of total ARX.
RRID:
AB_2837025
Cite Format: Affinity Biosciences Cat# DF4674, RRID:AB_2837025.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Aristaless related homeobox gene; Aristaless-related homeobox; Arx; ARX_HUMAN; Homeobox protein ARX; ISSX; MRX29; MRX32; MRX33; MRX36; MRX38; MRX43; MRX54; MRXS1; phox2a; PRTS;

Immunogens

Immunogen:
Uniprot:

Q96QS3(ARX_HUMAN) >>Visit HPA database.

Gene(ID):
ARX(170302)
Expression:
Q96QS3 ARX_HUMAN:

Expressed predominantly in fetal and adult brain and skeletal muscle. Expression is specific to the telencephalon and ventral thalamus. There is an absence of expression in the cerebellum throughout development and also in adult.

Sequence:
MSNQYQEEGCSERPECKSKSPTLLSSYCIDSILGRRSPCKMRLLGAAQSLPAPLTSRADPEKAVQGSPKSSSAPFEAELHLPPKLRRLYGPGGGRLLQGAAAAAAAAAAAAAAAATATAGPRGEAPPPPPPTARPGERPDGAGAAAAAAAAAAAAWDTLKISQAPQVSISRSKSYRENGAPFVPPPPALDELGGPGGVTHPEERLGVAGGPGSAPAAGGGTGTEDDEEELLEDEEDEDEEEELLEDDEEELLEDDARALLKEPRRCPVAATGAVAAAAAAAVATEGGELSPKEELLLHPEDAEGKDGEDSVCLSAGSDSEEGLLKRKQRRYRTTFTSYQLEELERAFQKTHYPDVFTREELAMRLDLTEARVQVWFQNRRAKWRKREKAGAQTHPPGLPFPGPLSATHPLSPYLDASPFPPHHPALDSAWTAAAAAAAAAFPSLPPPPGSASLPPSGAPLGLSTFLGAAVFRHPAFISPAFGRLFSTMAPLTSASTAAALLRQPTPAVEGAVASGALADPATAAADRRASSIAALRLKAKEHAAQLTQLNILPGTSTGKEVC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
91
Xenopus
78
Horse
0
Bovine
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96QS3 As Substrate

Site PTM Type Enzyme
R86 Methylation
R87 Methylation
Y89 Phosphorylation
R122 Methylation
S486 Phosphorylation

Research Backgrounds

Function:

Transcription factor required for normal brain development. May be important for maintenance of specific neuronal subtypes in the cerebral cortex and axonal guidance in the floor plate.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed predominantly in fetal and adult brain and skeletal muscle. Expression is specific to the telencephalon and ventral thalamus. There is an absence of expression in the cerebellum throughout development and also in adult.

Family&Domains:

Belongs to the paired homeobox family. Bicoid subfamily.

References

1). In utero exposure to phenanthrene induced islet cell dysfunction in adult mice: sex differences in the effects and potential causes. Science of The Total Environment, 2021 (PubMed: 33513515) [IF=8.2]

Application: WB    Species: mice    Sample: islet cell

Fig. 4. The expression of pancreatic proteins that participate in islet cell function of adult F1 mice. The data were shown as the mean ± SE and analyzed with SPSS 19.0 software. Significant difference (p < 0.05) was tested using one-way ANOVA (Duncan's test), which are labeled by *. n = 4, 3 dams from each group, and 1–2 F1 mice from each dam.
2). Exposure to Aroclor 1254 differentially affects the survival of pancreatic β-cells and α-cells in the male mice and the potential reason. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2020 (PubMed: 31706244) [IF=6.2]

Application: IF/ICC    Species: mouse    Sample: islet cell

Fig. 6.| Immunofluorescence analysis of Pdx1 and Arx expression in islet cells of male mice exposed to Aroclor 1254 for 60 days. (A) Representative images of triple staining for insulin (green), Pdx-1 (red), and DAPI (blue) on fixed, paraffin-embedded pancreas sections. (B) Percentage of Pdx-1 positive data are presented as mean ± SE (n = 3). (C) Representative images of triple staining for glucagon (green), Arx (red), and DAPI (blue) on fixed, paraffin-embedded pancreas sections. (D)Percentage of Arx positive data are presented as mean ± SE (n = 3). Means of exposures not sharing a common letter are significantly different at P < 0.05 as assessed by one-way ANOVA followed by the Duncan test. Scale bar = 50 μm.

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