Product: THOC4 Antibody
Catalog: DF4664
Description: Rabbit polyclonal antibody to THOC4
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 27 KD; 27kD(Calculated).
Uniprot: Q86V81
RRID: AB_2837015

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
THOC4 Antibody detects endogenous levels of total THOC4.
RRID:
AB_2837015
Cite Format: Affinity Biosciences Cat# DF4664, RRID:AB_2837015.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ally of AML-1 and LEF-1; Ally of AML1 and LEF1; ALY; ALY/REF; Aly/REF export factor; BEF; bZIP enhancing factor; bZIP-enhancing factor BEF; REF; THO complex 4; THO complex subunit 4; Tho4; thoc4; THOC4_HUMAN; Transcriptional coactivator Aly/REF; Transcriptional coactivator;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q86V81 THOC4_HUMAN:

Expressed in a wide variety of cancer types.

Sequence:
MADKMDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPAIARGAAGGGGRNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNRGGMTRNRGAGGFGGGGGTRRGTRGGARGRGRGAGRNSKQQLSAEELDAQLDAYNARMDTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86V81 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K4 Acetylation
K4 Ubiquitination
S8 Phosphorylation
K14 Ubiquitination
S18 Phosphorylation
R31 Methylation
S34 Phosphorylation P31749 (AKT1)
R38 Methylation
R47 Methylation
R50 Methylation
R56 Methylation
R58 Methylation
R63 Methylation
R71 Methylation
R73 Methylation
Y77 Phosphorylation
S78 Phosphorylation
K81 Ubiquitination
K86 Acetylation
K86 Ubiquitination
S94 Phosphorylation
T104 Phosphorylation
K134 Ubiquitination
R141 Methylation
S142 Phosphorylation
S145 Phosphorylation
T148 Phosphorylation
K161 Ubiquitination
K164 Ubiquitination
S183 Phosphorylation
R190 Methylation
R197 Methylation
R202 Methylation
R204 Methylation
T215 Phosphorylation
R216 Methylation
T219 Phosphorylation P31749 (AKT1)
K235 Acetylation
K235 Methylation
K235 Ubiquitination
S239 Phosphorylation
Y250 Phosphorylation
T256 Phosphorylation
S257 Phosphorylation

Research Backgrounds

Function:

Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability. Involved in mRNA export of C5-methylcytosine (m5C)-containing mRNAs: specifically recognizes and binds m5C mRNAs and mediates their nucleo-cytoplasmic shuttling.

Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation.

PTMs:

Arg-204 is dimethylated, probably to asymmetric dimethylarginine. Arginine methylation reduces RNA binding.

Citrullinated by PADI4.

Subcellular Location:

Nucleus. Nucleus speckle. Cytoplasm.
Note: Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA (PubMed:19324961). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a wide variety of cancer types.

Subunit Structure:

Homomultimer. Is part of several complexes involved in mRNA processing and export. Component of the transcription/export (TREX) complex at least composed of ALYREF/THOC4, DDX39B, SARNP/CIP29, CHTOP and the THO subcomplex; TREX seems to have a dynamic structure involving ATP-dependent remodeling; in the complex interacts (via C-terminus) directly with DDX39B and interacts directly with THOC1 and THOC2. Found in mRNA splicing-dependent exon junction complexes (EJC). Identified in the spliceosome C complex. Found in a mRNP complex with UPF3A and UPF3B. Interacts with RBM8A, NCBP1, THOC5, LEF1, RUNX1, EIF4A3, RNPS1, SRRM1, IWS1 and EXOSC1. Interacts with RBM15B. Interacts with NXF1; the interaction is direct.

(Microbial infection) Interacts with human Kaposi's sarcoma-associated herpesvirus (HHV-8) ORF57 protein; this interaction allows efficient export of HHV-8 early and late intronless transcripts.

(Microbial infection) Interacts with HHV-1 ICP27 protein; this interaction recruits ALYREF to viral replication compartments and probably directs viral mRNA to the TAP/NFX1 pathway.

Family&Domains:

Belongs to the ALYREF family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Transcription > Spliceosome.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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