MED27 Antibody - #DF4660

(1)  
Product: MED27 Antibody
Catalog: DF4660
Description: Rabbit polyclonal antibody to MED27
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 35 KD; 35kD(Calculated).
Uniprot: Q6P2C8
RRID: AB_2837011

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
MED27 Antibody detects endogenous levels of total MED27.
RRID:
AB_2837011
Cite Format: Affinity Biosciences Cat# DF4660, RRID:AB_2837011.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cofactor required for Sp1 transcriptional activation subunit 8 (34kD); Cofactor required for Sp1 transcriptional activation subunit 8 34kDa; Cofactor required for Sp1 transcriptional activation subunit 8; CRAP 34; CRAP34; CRSP 34; CRSP 8; CRSP complex subunit 8; CRSP34; CRSP8; MED 27; Med27; MED27_HUMAN; Mediator complex subunit 27; Mediator of RNA polymerase II transcription subunit 27; MGC11274; P37 TRAP/SMCC/PC2 subunit; Transcriptional coactivator CRSP34; TRAP 37; TRAP37;

Immunogens

Immunogen:
Uniprot:

Q6P2C8(MED27_HUMAN) >>Visit HPA database.

Gene(ID):
MED27(9442)
Sequence:
MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
89
Zebrafish
89
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6P2C8 As Substrate

Site PTM Type Enzyme
S27 Phosphorylation
S29 Phosphorylation
K36 Ubiquitination
K42 Ubiquitination
K50 Ubiquitination
K79 Ubiquitination
K100 Ubiquitination
Y104 Phosphorylation
K112 Ubiquitination
K116 Ubiquitination
S132 Phosphorylation
K134 Methylation
K134 Ubiquitination
K145 Acetylation
K145 Ubiquitination
S177 Phosphorylation
S181 Phosphorylation
K215 Ubiquitination
Y222 Phosphorylation
K227 Ubiquitination
S232 Phosphorylation
K233 Ubiquitination
S234 Phosphorylation
Y236 Phosphorylation
T243 Phosphorylation
K275 Ubiquitination

Research Backgrounds

Function:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.

Family&Domains:

Belongs to the Mediator complex subunit 27 family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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