ZFYVE19 Antibody - #DF4625

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Product: ZFYVE19 Antibody
Catalog: DF4625
Description: Rabbit polyclonal antibody to ZFYVE19
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 50 KD; 52kD(Calculated).
Uniprot: Q96K21
RRID: AB_2836989

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Horse(87%), Rabbit(100%), Dog(86%)
Clonality:
Polyclonal
Specificity:
ZFYVE19 Antibody detects endogenous levels of total ZFYVE19.
RRID:
AB_2836989
Cite Format: Affinity Biosciences Cat# DF4625, RRID:AB_2836989.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Abscission/NoCut checkpoint regulator; ANCHR; MLL partner containing FYVE domain; MPFYVE; ZFY19_HUMAN; ZFYVE 19; Zfyve19; Zinc finger FYVE domain containing protein 19; Zinc finger FYVE domain-containing protein 19; Zinc finger FYVE type containing 19; Zinc finger, FYVE domain containing 19;

Immunogens

Immunogen:
Uniprot:

Q96K21(ANCHR_HUMAN) >>Visit HPA database.

Gene(ID):
ZFYVE19(84936)
Expression:
Q96K21 ANCHR_HUMAN:

Detected in brain, heart, skeletal muscle, kidney and liver.

Sequence:
MNYDSQQPPLPPLPYAGCRRASGFPALGRGGTVPVGVWGGAGQGREGRSWGEGPRGPGLGRRDLSSADPAVLGATMESRCYGCAVKFTLFKKEYGCKNCGRAFCSGCLSFSAAVPRTGNTQQKVCKQCHEVLTRGSSANASKWSPPQNYKKRVAALEAKQKPSTSQSQGLTRQDQMIAERLARLRQENKPKLVPSQAEIEARLAALKDERQGSIPSTQEMEARLAALQGRVLPSQTPQPAHHTPDTRTQAQQTQDLLTQLAAEVAIDESWKGGGPAASLQNDLNQGGPGSTNSKRQANWSLEEEKSRLLAEAALELREENTRQERILALAKRLAMLRGQDPERVTLQDYRLPDSDDDEDEETAIQRVLQQLTEEASLDEASGFNIPAEQASRPWTQPRGAEPEAQDVDPRPEAEEEELPWCCICNEDATLRCAGCDGDLFCARCFREGHDAFELKEHQTSAYSPPRAGQEH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
87
Dog
86
Pig
79
Bovine
79
Sheep
79
Chicken
73
Xenopus
47
Zebrafish
40
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96K21 As Substrate

Site PTM Type Enzyme
Y3 Phosphorylation
S5 Phosphorylation
Y15 Phosphorylation
S22 Phosphorylation
S105 Phosphorylation
K126 Ubiquitination
S141 Phosphorylation
S144 Phosphorylation
K159 Ubiquitination
K161 Ubiquitination
K207 Sumoylation
K207 Ubiquitination
S213 Phosphorylation
S216 Phosphorylation
T217 Phosphorylation
T236 Phosphorylation
T243 Phosphorylation
S278 Phosphorylation
S290 Phosphorylation
S293 Phosphorylation
K294 Ubiquitination
S300 Phosphorylation
K305 Ubiquitination
K331 Ubiquitination
T345 Phosphorylation
Y349 Phosphorylation
S354 Phosphorylation
S376 Phosphorylation
T459 Phosphorylation
S460 Phosphorylation
S463 Phosphorylation

Research Backgrounds

Function:

Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cleavage furrow. Midbody>Midbody ring.
Note: Localizes mainly on centrosomes in interphase and early mitosis. Localizes at the cleavage furrow and midbody ring in late mitosis and cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in brain, heart, skeletal muscle, kidney and liver.

Subunit Structure:

Interacts (via MIM1-B) with VPS4A; interaction takes place at the midbody ring following cytokinesis checkpoint activation.

Family&Domains:

The FYVE-type zinc finger mediates binding to phosphatidylinositol-3-phosphate (PtdIns(3)P).

The MIM1-B motif mediates interaction with VPS4A.

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