ZC3H13 Antibody - #DF4623

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Product: ZC3H13 Antibody
Catalog: DF4623
Description: Rabbit polyclonal antibody to ZC3H13
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 200 KD; 197kD(Calculated).
Uniprot: Q5T200
RRID: AB_2836987

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
ZC3H13 Antibody detects endogenous levels of total ZC3H13.
RRID:
AB_2836987
Cite Format: Affinity Biosciences Cat# DF4623, RRID:AB_2836987.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2600010B19Rik; 3110050K21Rik; 4930570G11Rik; C87618; DKFZp434D1812; FLJ35669; KIAA0853; ZC3H13; ZC3HD_HUMAN; Zinc finger CCCH domain-containing protein 13; zinc finger CCCH type containing 13;

Immunogens

Immunogen:
Uniprot:

Q5T200(ZC3HD_HUMAN) >>Visit HPA database.

Gene(ID):
ZC3H13(23091)
Sequence:
MSKIRRKVTVENTKTISDSTSRRPSVFERLGPSTGSTAETQCRNWLKTGNCLYGNTCRFVHGPSPRGKGYSSNYRRSPERPTGDLRERMKNKRQDVDTEPQKRNTEESSSPVRKESSRGRHREKEDIKITKERTPESEEENVEWETNRDDSDNGDINYDYVHELSLEMKRQKIQRELMKLEQENMEKREEIIIKKEVSPEVVRSKLSPSPSLRKSSKSPKRKSSPKSSSASKKDRKTSAVSSPLLDQQRNSKTNQSKKKGPRTPSPPPPIPEDIALGKKYKEKYKVKDRIEEKTRDGKDRGRDFERQREKRDKPRSTSPAGQHHSPISSRHHSSSSQSGSSIQRHSPSPRRKRTPSPSYQRTLTPPLRRSASPYPSHSLSSPQRKQSPPRHRSPMREKGRHDHERTSQSHDRRHERREDTRGKRDREKDSREEREYEQDQSSSRDHRDDREPRDGRDRRDARDTRDRRELRDSRDMRDSREMRDYSRDTKESRDPRDSRSTRDAHDYRDREGRDTHRKEDTYPEESRSYGRNHLREESSRTEIRNESRNESRSEIRNDRMGRSRGRVPELPEKGSRGSRGSQIDSHSSNSNYHDSWETRSSYPERDRYPERDNRDQARDSSFERRHGERDRRDNRERDQRPSSPIRHQGRNDELERDERREERRVDRVDDRRDERARERDRERERDRERERERERERDREREKERELERERAREREREREKERDRERDRDRDHDRERERERERDREKEREREREERERERERERERERERERERERARERDKERERQRDWEDKDKGRDDRREKREEIREDRNPRDGHDERKSKKRYRNEGSPSPRQSPKRRREHSPDSDAYNSGDDKNEKHRLLSQVVRPQESRSLSPSHLTEDRQGRWKEEDRKPERKESSRRYEEQELKEKVSSVDKQREQTEILESSRMRAQDIIGHHQSEDRETSDRAHDENKKKAKIQKKPIKKKKEDDVGIERGNIETTSEDGQVFSPKKGQKKKSIEKKRKKSKGDSDISDEEAAQQSKKKRGPRTPPITTKEELVEMCNGKNGILEDSQKKEDTAFSDWSDEDVPDRTEVTEAEHTATATTPGSTPSPLSSLLPPPPPVATATATTVPATLAATTAAAATSFSTSAITISTSATPTNTTNNTFANEDSHRKCHRTRVEKVETPHVTIEDAQHRKPMDQKRSSSLGSNRSNRSHTSGRLRSPSNDSAHRSGDDQSGRKRVLHSGSRDREKTKSLEITGERKSRIDQLKRGEPSRSTSSDRQDSRSHSSRRSSPESDRQVHSRSGSFDSRDRLQERDRYEHDRERERERRDTRQREWDRDADKDWPRNRDRDRLRERERERERDKRRDLDRERERLISDSVERDRDRDRDRTFESSQIESVKRCEAKLEGEHERDLESTSRDSLALDKERMDKDLGSVQGFEETNKSERTESLEGDDESKLDDAHSLGSGAGEGYEPISDDELDEILAGDAEKREDQQDEEKMPDPLDVIDVDWSGLMPKHPKEPREPGAALLKFTPGAVMLRVGISKKLAGSELFAKVKETCQRLLEKPKDADNLFEHELGALNMAALLRKEERASLLSNLGPCCKALCFRRDSAIRKQLVKNEKGTIKQAYTSAPMVDNELLRLSLRLFKRKTTCHAPGHEKTEDNKLSQSSIQQELCVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
83
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5T200 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
S17 Phosphorylation
S25 Phosphorylation
K47 Methylation
Y53 Phosphorylation
S64 Phosphorylation
R66 Methylation
K68 Methylation
S77 Phosphorylation
T82 Phosphorylation
S108 Phosphorylation
S109 Phosphorylation
S110 Phosphorylation
T134 Phosphorylation
S137 Phosphorylation
S151 Phosphorylation
Y158 Phosphorylation
Y160 Phosphorylation
K194 Sumoylation
S198 Phosphorylation
S204 Phosphorylation
S207 Phosphorylation
S209 Phosphorylation
S211 Phosphorylation
S216 Phosphorylation
S218 Phosphorylation
S223 Phosphorylation
T237 Phosphorylation
S238 Phosphorylation
S241 Phosphorylation
S242 Phosphorylation
T263 Phosphorylation
S265 Phosphorylation
S316 Phosphorylation
T317 Phosphorylation
S318 Phosphorylation
S325 Phosphorylation
S328 Phosphorylation
S329 Phosphorylation
S333 Phosphorylation
S334 Phosphorylation
S335 Phosphorylation
S336 Phosphorylation
S338 Phosphorylation
S341 Phosphorylation
S346 Phosphorylation
S348 Phosphorylation
T354 Phosphorylation
S356 Phosphorylation
S358 Phosphorylation
Y359 Phosphorylation
T364 Phosphorylation
S370 Phosphorylation
S372 Phosphorylation
Y374 Phosphorylation
S376 Phosphorylation
S378 Phosphorylation
S380 Phosphorylation
S381 Phosphorylation
S387 Phosphorylation
S393 Phosphorylation
T406 Phosphorylation
S430 Phosphorylation
Y436 Phosphorylation
S473 Phosphorylation
S479 Phosphorylation
S492 Phosphorylation
S538 Phosphorylation
S539 Phosphorylation
T541 Phosphorylation
S563 Phosphorylation
S578 Phosphorylation
S581 Phosphorylation
Y592 Phosphorylation
S595 Phosphorylation
S600 Phosphorylation
Y608 Phosphorylation
S620 Phosphorylation
S621 Phosphorylation
S642 Phosphorylation
S643 Phosphorylation
S831 Phosphorylation
S833 Phosphorylation
S837 Phosphorylation
S845 Phosphorylation
S848 Phosphorylation
Y851 Phosphorylation
S853 Phosphorylation
S865 Phosphorylation
S873 Phosphorylation
S875 Phosphorylation
S877 Phosphorylation
S879 Phosphorylation
T882 Phosphorylation
S943 Phosphorylation
S949 Phosphorylation
T984 Phosphorylation
T985 Phosphorylation
S986 Phosphorylation
S993 Phosphorylation
S1010 Phosphorylation
S1014 Phosphorylation
S1017 Phosphorylation
T1033 Phosphorylation
S1056 Phosphorylation
S1065 Phosphorylation
S1068 Phosphorylation
T1170 Phosphorylation
S1190 Phosphorylation
S1191 Phosphorylation
S1194 Phosphorylation
S1197 Phosphorylation
S1208 Phosphorylation
S1210 Phosphorylation
S1213 Phosphorylation
S1217 Phosphorylation
S1230 Phosphorylation
S1232 Phosphorylation
S1240 Phosphorylation
T1244 Phosphorylation
S1274 Phosphorylation
S1279 Phosphorylation
S1282 Phosphorylation
S1288 Phosphorylation
S1290 Phosphorylation
S1292 Phosphorylation
S1295 Phosphorylation
T1318 Phosphorylation
S1364 Phosphorylation
S1366 Phosphorylation
S1382 Phosphorylation
S1386 Phosphorylation
T1405 Phosphorylation
S1406 Phosphorylation
S1409 Phosphorylation
S1423 Phosphorylation
S1433 Phosphorylation
T1436 Phosphorylation
S1438 Phosphorylation
S1445 Phosphorylation
S1452 Phosphorylation
S1455 Phosphorylation
Y1461 Phosphorylation
S1465 Phosphorylation
T1522 Phosphorylation
K1557 Ubiquitination
K1593 Ubiquitination
S1601 Phosphorylation
K1616 Ubiquitination
S1657 Phosphorylation
S1659 Phosphorylation
S1660 Phosphorylation

Research Backgrounds

Function:

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs at the 3'-UTR (By similarity). Controls embryonic stem cells (ESCs) pluripotency via its role in m6A methylation (By similarity). In the WMM complex, anchors component of the MACOM subcomplex in the nucleus (By similarity). Also required for bridging WTAP to the RNA-binding component RBM15 (RBM15 or RBM15B) (By similarity).

Subcellular Location:

Nucleus speckle. Nucleus>Nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM. The MAC subcomplex is composed of METTL3 and METTL14. The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B). Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, RBM15, BCLAF1 and THRAP3.

Family&Domains:

Belongs to the ZC3H13 family.

References

1). A benchmark study of deep learning-based multi-omics data fusion methods for cancer. GENOME BIOLOGY, 2022 (PubMed: 35945544) [IF=10.1]
2). Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop. Journal of Ginseng Research, 2021 (PubMed: 34764728) [IF=6.8]

Application: WB    Species: Human    Sample: MDA-MB-157 (top), NMFH-1 (middle), and TT (bottom) cells

Fig. 2 KIF26B interacts with ZC3H13 and CBLL1 in the cytoplasmic fraction of multiple cancer cell lines. A. IF staining of KIF26 (red), CBLL1 (green), and ZC3H13 (green) in MDA-MB-157, NMFH-1 and TT cells. B. Left panel, Co-IP was conducted using the cytoplasmic fraction MDA-MB-157 (top), NMFH-1 (middle), and TT (bottom) cells. Right panel, reciprocal IP assay was performed to explore the interaction of ZC3H13 and CBLL1 with KIF26B. KIF26B was immunoprecipitated by anti-CBLL1 and anti-C3H13. C and D. The structure of the wild-type full length (FL) and mutation constructs of Flag-tag labeled ZC3H13 (C) and CBLL1 (D). E and F. Flag-tagged FL or truncated mutation constructs were co-transfected with HA-KIF26B into NMFH-1 cells. The KIF26B/Flag-tag complexes were immunoprecipitated by anti-HA antibodies. Flag-ZC3H13 (E) and Flag-CBLL1 (F) proteins were detected using anti-Flag antibodies.

Application: IF/ICC    Species: Human    Sample: MDA-MB-157 (top), NMFH-1 (middle), and TT (bottom) cells

Fig. 2 KIF26B interacts with ZC3H13 and CBLL1 in the cytoplasmic fraction of multiple cancer cell lines. A. IF staining of KIF26 (red), CBLL1 (green), and ZC3H13 (green) in MDA-MB-157, NMFH-1 and TT cells. B. Left panel, Co-IP was conducted using the cytoplasmic fraction MDA-MB-157 (top), NMFH-1 (middle), and TT (bottom) cells. Right panel, reciprocal IP assay was performed to explore the interaction of ZC3H13 and CBLL1 with KIF26B. KIF26B was immunoprecipitated by anti-CBLL1 and anti-C3H13. C and D. The structure of the wild-type full length (FL) and mutation constructs of Flag-tag labeled ZC3H13 (C) and CBLL1 (D). E and F. Flag-tagged FL or truncated mutation constructs were co-transfected with HA-KIF26B into NMFH-1 cells. The KIF26B/Flag-tag complexes were immunoprecipitated by anti-HA antibodies. Flag-ZC3H13 (E) and Flag-CBLL1 (F) proteins were detected using anti-Flag antibodies.

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