WASF3 Antibody - #DF4619

(3)  
Product: WASF3 Antibody
Catalog: DF4619
Description: Rabbit polyclonal antibody to WASF3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 55 KD; 55kD(Calculated).
Uniprot: Q9UPY6
RRID: AB_2836983

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
WASF3 Antibody detects endogenous levels of total WASF3.
RRID:
AB_2836983
Cite Format: Affinity Biosciences Cat# DF4619, RRID:AB_2836983.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA0900; Protein WAVE-3; Protein WAVE3; SCAR3; Verprolin homology domain containing protein 3; Verprolin homology domain-containing protein 3; WASF3; WASF3_HUMAN; WASP family protein member 3; WAVE3; Wiskott Aldrich syndrome protein family member 3; Wiskott-Aldrich syndrome protein family member 3;

Immunogens

Immunogen:
Uniprot:

Q9UPY6(WASF3_HUMAN) >>Visit HPA database.

Gene(ID):
WASF3(10810)
Expression:
Q9UPY6 WASF3_HUMAN:

Expressed in ovary and brain.

Sequence:
MPLVKRNIEPRHLCRGALPEGITSELECVTNSTLAAIIRQLSSLSKHAEDIFGELFNEANNFYIRANSLQDRIDRLAVKVTQLDSTVEEVSLQDINMKKAFKSSTVQDQQVVSKNSIPNPVADIYNQSDKPPPLNILTPYRDDKKDGLKFYTDPSYFFDLWKEKMLQDTEDKRKEKRRQKEQKRIDGTTREVKKVRKARNRRQEWNMMAYDKELRPDNRLSQSVYHGASSEGSLSPDTRSHASDVTDYSYPATPNHSLHPQPVTPSYAAGDVPPHGPASQAAEHEYRPPSASARHMALNRPQQPPPPPPPQAPEGSQASAPMAPADYGMLPAQIIEYYNPSGPPPPPPPPVIPSAQTAFVSPLQMPMQPPFPASASSTHAAPPHPPSTGLLVTAPPPPGPPPPPPGPPGPGSSLSSSPMHGPPVAEAKRQEPAQPPISDARSDLLAAIRMGIQLKKVQEQREQEAKREPVGNDVATILSRRIAVEYSDSDDDSEFDENDWSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
90
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UPY6 As Substrate

Site PTM Type Enzyme
Y151 Phosphorylation P00519 (ABL1)
Y156 Phosphorylation
K162 Ubiquitination
T169 Phosphorylation
S223 Phosphorylation
Y225 Phosphorylation
S235 Phosphorylation
T246 Phosphorylation
Y248 Phosphorylation P00519 (ABL1)
Y286 Phosphorylation
Y337 Phosphorylation P00519 (ABL1)
Y486 Phosphorylation P00519 (ABL1)

Research Backgrounds

Function:

Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.

PTMs:

Phosphorylation by ABL1 promotes lamellipodia formation and cell migration.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in ovary and brain.

Subunit Structure:

Binds actin and the Arp2/3 complex.

Family&Domains:

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Belongs to the SCAR/WAVE family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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